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Title: 2D IR spectroscopy reveals the role of water in the binding of channel-blocking drugs to the influenza M2 channel

Abstract

Water is an integral part of the homotetrameric M2 proton channel of the influenza A virus, which not only assists proton conduction but could also play an important role in stabilizing channel-blocking drugs. Herein, we employ two dimensional infrared (2D IR) spectroscopy and site-specific IR probes, i.e., the amide I bands arising from isotopically labeled Ala30 and Gly34 residues, to probe how binding of either rimantadine or 7,7-spiran amine affects the water dynamics inside the M2 channel. Our results show, at neutral pH where the channel is non-conducting, that drug binding leads to a significant increase in the mobility of the channel water. A similar trend is also observed at pH 5.0 although the difference becomes smaller. Taken together, these results indicate that the channel water facilitates drug binding by increasing its entropy. Furthermore, the 2D IR spectral signatures obtained for both probes under different conditions collectively support a binding mechanism whereby amantadine-like drugs dock in the channel with their ammonium moiety pointing toward the histidine residues and interacting with a nearby water cluster, as predicted by molecular dynamics simulations. We believe these findings have important implications for designing new anti-influenza drugs.

Authors:
; ;  [1]; ;  [2]; ;  [3];  [4]
  1. Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104 (United States)
  2. Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California 94143 (United States)
  3. Department of Chemistry, Syracuse University, Syracuse, New York 13244 (United States)
  4. Department of Chemistry, University of Wisconsin, Madison, Wisconsin 53706 (United States)
Publication Date:
OSTI Identifier:
22420130
Resource Type:
Journal Article
Journal Name:
Journal of Chemical Physics
Additional Journal Information:
Journal Volume: 140; Journal Issue: 23; Other Information: (c) 2014 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0021-9606
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; AMINES; ENTROPY; HISTIDINE; INFLUENZA; INFRARED SPECTRA; MOLECULAR DYNAMICS METHOD; PROBES; PROTONS; SIMULATION; VIRUSES; WATER

Citation Formats

Ghosh, Ayanjeet, Gai, Feng, Hochstrasser, Robin M., Wang, Jun, DeGrado, William F., Moroz, Yurii S., Korendovych, Ivan V., and Zanni, Martin. 2D IR spectroscopy reveals the role of water in the binding of channel-blocking drugs to the influenza M2 channel. United States: N. p., 2014. Web. doi:10.1063/1.4881188.
Ghosh, Ayanjeet, Gai, Feng, Hochstrasser, Robin M., Wang, Jun, DeGrado, William F., Moroz, Yurii S., Korendovych, Ivan V., & Zanni, Martin. 2D IR spectroscopy reveals the role of water in the binding of channel-blocking drugs to the influenza M2 channel. United States. https://doi.org/10.1063/1.4881188
Ghosh, Ayanjeet, Gai, Feng, Hochstrasser, Robin M., Wang, Jun, DeGrado, William F., Moroz, Yurii S., Korendovych, Ivan V., and Zanni, Martin. 2014. "2D IR spectroscopy reveals the role of water in the binding of channel-blocking drugs to the influenza M2 channel". United States. https://doi.org/10.1063/1.4881188.
@article{osti_22420130,
title = {2D IR spectroscopy reveals the role of water in the binding of channel-blocking drugs to the influenza M2 channel},
author = {Ghosh, Ayanjeet and Gai, Feng and Hochstrasser, Robin M. and Wang, Jun and DeGrado, William F. and Moroz, Yurii S. and Korendovych, Ivan V. and Zanni, Martin},
abstractNote = {Water is an integral part of the homotetrameric M2 proton channel of the influenza A virus, which not only assists proton conduction but could also play an important role in stabilizing channel-blocking drugs. Herein, we employ two dimensional infrared (2D IR) spectroscopy and site-specific IR probes, i.e., the amide I bands arising from isotopically labeled Ala30 and Gly34 residues, to probe how binding of either rimantadine or 7,7-spiran amine affects the water dynamics inside the M2 channel. Our results show, at neutral pH where the channel is non-conducting, that drug binding leads to a significant increase in the mobility of the channel water. A similar trend is also observed at pH 5.0 although the difference becomes smaller. Taken together, these results indicate that the channel water facilitates drug binding by increasing its entropy. Furthermore, the 2D IR spectral signatures obtained for both probes under different conditions collectively support a binding mechanism whereby amantadine-like drugs dock in the channel with their ammonium moiety pointing toward the histidine residues and interacting with a nearby water cluster, as predicted by molecular dynamics simulations. We believe these findings have important implications for designing new anti-influenza drugs.},
doi = {10.1063/1.4881188},
url = {https://www.osti.gov/biblio/22420130}, journal = {Journal of Chemical Physics},
issn = {0021-9606},
number = 23,
volume = 140,
place = {United States},
year = {Sat Jun 21 00:00:00 EDT 2014},
month = {Sat Jun 21 00:00:00 EDT 2014}
}