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Title: Aculeatin, a coumarin derived from Toddalia asiatica (L.) Lam., enhances differentiation and lipolysis of 3T3-L1 adipocytes

Abstract

Highlights: • Aculeatin promoted adipocyte differentiation. • Aculeatin improved glucose uptake. • Aculeatin enhanced adipocyte lipolysis. - Abstract: Toddalia asiatica (L.) Lam. (T. asiatica) has been utilized traditionally for medicinal purposes such as the treatment of diabetes. Currently, the extract is considered to be a good source of anti-diabetic agents, but the active compounds have yet to be identified. In this study, we investigated the effects of fractionated T. asiatica extracts on the differentiation of 3T3-L1 preadipocytes and identified aculeatin as a potential active agent. When 3T3-L1 preadipocytes were treated with aculeatin isolated from T. asiatica in the presence of insulin, aculeatin increased cellular triglyceride levels and glycerol-3-phosphate dehydrogenase activity. This indicated that aculeatin could enhance the differentiation of preadipocytes into adipocytes. Further analyses using a DNA microarray and real-time quantitative reverse-transcription PCR showed an increase in the expression of peroxisome proliferator-activated receptor-γ target genes (Pparg, Ap2, Cd36, Glut4 and Adipoq) by aculeatin, suggesting that aculeatin enhances the differentiation of 3T3-L1 cells by modulating the expression of genes critical for adipogenesis. Interestingly, after treatment of differentiated adipocytes with aculeatin, glucose uptake and lipolysis were enhanced. Overall, our results suggested that aculeatin is an active compound in T. asiatica for enhancingmore » both differentiation and lipolysis of adipocytes, which are useful for the treatment of lipid abnormalities as well as diabetes.« less

Authors:
 [1];  [2]; ; ; ; ; ; ;  [1]; ;  [3]
  1. Japan Food Research Laboratories, Osaka 567-0085 (Japan)
  2. (Japan)
  3. Food and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University, Miyagi 981-8555 (Japan)
Publication Date:
OSTI Identifier:
22416815
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 453; Journal Issue: 4; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ACETATES; ACETONITRILE; ALBUMINS; COUMARIN; DMSO; DNA; EDTA; GENES; GLUCOSE; GLYCEROL; HEXANE; INSULIN; MASS SPECTROSCOPY; METHANOL; MONOCYTES; NUCLEAR MAGNETIC RESONANCE; POLYMERASE CHAIN REACTION; RECEPTORS; TRANSCRIPTION; TRIGLYCERIDES

Citation Formats

Watanabe, Akio, E-mail: watanabea@jfrl.or.jp, Food and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University, Miyagi 981-8555, Kato, Tsuyoshi, Ito, Yusuke, Yoshida, Izumi, Harada, Teppei, Mishima, Takashi, Fujita, Kazuhiro, Watai, Masatoshi, Nakagawa, Kiyotaka, and Miyazawa, Teruo. Aculeatin, a coumarin derived from Toddalia asiatica (L.) Lam., enhances differentiation and lipolysis of 3T3-L1 adipocytes. United States: N. p., 2014. Web. doi:10.1016/J.BBRC.2014.10.027.
Watanabe, Akio, E-mail: watanabea@jfrl.or.jp, Food and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University, Miyagi 981-8555, Kato, Tsuyoshi, Ito, Yusuke, Yoshida, Izumi, Harada, Teppei, Mishima, Takashi, Fujita, Kazuhiro, Watai, Masatoshi, Nakagawa, Kiyotaka, & Miyazawa, Teruo. Aculeatin, a coumarin derived from Toddalia asiatica (L.) Lam., enhances differentiation and lipolysis of 3T3-L1 adipocytes. United States. doi:10.1016/J.BBRC.2014.10.027.
Watanabe, Akio, E-mail: watanabea@jfrl.or.jp, Food and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University, Miyagi 981-8555, Kato, Tsuyoshi, Ito, Yusuke, Yoshida, Izumi, Harada, Teppei, Mishima, Takashi, Fujita, Kazuhiro, Watai, Masatoshi, Nakagawa, Kiyotaka, and Miyazawa, Teruo. Fri . "Aculeatin, a coumarin derived from Toddalia asiatica (L.) Lam., enhances differentiation and lipolysis of 3T3-L1 adipocytes". United States. doi:10.1016/J.BBRC.2014.10.027.
@article{osti_22416815,
title = {Aculeatin, a coumarin derived from Toddalia asiatica (L.) Lam., enhances differentiation and lipolysis of 3T3-L1 adipocytes},
author = {Watanabe, Akio, E-mail: watanabea@jfrl.or.jp and Food and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University, Miyagi 981-8555 and Kato, Tsuyoshi and Ito, Yusuke and Yoshida, Izumi and Harada, Teppei and Mishima, Takashi and Fujita, Kazuhiro and Watai, Masatoshi and Nakagawa, Kiyotaka and Miyazawa, Teruo},
abstractNote = {Highlights: • Aculeatin promoted adipocyte differentiation. • Aculeatin improved glucose uptake. • Aculeatin enhanced adipocyte lipolysis. - Abstract: Toddalia asiatica (L.) Lam. (T. asiatica) has been utilized traditionally for medicinal purposes such as the treatment of diabetes. Currently, the extract is considered to be a good source of anti-diabetic agents, but the active compounds have yet to be identified. In this study, we investigated the effects of fractionated T. asiatica extracts on the differentiation of 3T3-L1 preadipocytes and identified aculeatin as a potential active agent. When 3T3-L1 preadipocytes were treated with aculeatin isolated from T. asiatica in the presence of insulin, aculeatin increased cellular triglyceride levels and glycerol-3-phosphate dehydrogenase activity. This indicated that aculeatin could enhance the differentiation of preadipocytes into adipocytes. Further analyses using a DNA microarray and real-time quantitative reverse-transcription PCR showed an increase in the expression of peroxisome proliferator-activated receptor-γ target genes (Pparg, Ap2, Cd36, Glut4 and Adipoq) by aculeatin, suggesting that aculeatin enhances the differentiation of 3T3-L1 cells by modulating the expression of genes critical for adipogenesis. Interestingly, after treatment of differentiated adipocytes with aculeatin, glucose uptake and lipolysis were enhanced. Overall, our results suggested that aculeatin is an active compound in T. asiatica for enhancing both differentiation and lipolysis of adipocytes, which are useful for the treatment of lipid abnormalities as well as diabetes.},
doi = {10.1016/J.BBRC.2014.10.027},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 4,
volume = 453,
place = {United States},
year = {2014},
month = {10}
}