Over-expression of Oct4 and Sox2 transcription factors enhances differentiation of human umbilical cord blood cells in vivo

Journal Article · Fri Sep 05 04:00:00 EDT 2014 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2014.07.132· OSTI ID:22416727

Guseva, Daria ^[1]; Rizvanov, Albert A.; Salafutdinov, Ilnur I.; Kudryashova, Nezhdana V. ^[2]; Palotás, András ^[2]; Asklepios-Med, Szeged ^[3]

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1. Kazan State Medical University, Kazan, Republic of Tatarstan (Russian Federation)
2. Kazan Federal University, Kazan, Republic of Tatarstan (Russian Federation)
3. Private Medical Practice and Research Center; Hungary

Highlights: • Gene and cell-based therapies comprise innovative aspects of regenerative medicine. • Genetically modified hUCB-MCs enhanced differentiation of cells in a mouse model of ALS. • Stem cells successfully transformed into micro-glial and endothelial lines in spinal cords. • Over-expressing oct4 and sox2 also induced production of neural marker PGP9.5. • Formation of new nerve cells, secreting trophic factors and neo-vascularisation could improve symptoms in ALS. - Abstract: Gene and cell-based therapies comprise innovative aspects of regenerative medicine. Even though stem cells represent a highly potential therapeutic strategy, their wide-spread exploitation is marred by ethical concerns, potential for malignant transformation and a plethora of other technical issues, largely restricting their use to experimental studies. Utilizing genetically modified human umbilical cord blood mono-nuclear cells (hUCB-MCs), this communication reports enhanced differentiation of transplants in a mouse model of amyotrophic lateral sclerosis (ALS). Over-expressing Oct4 and Sox2 induced production of neural marker PGP9.5, as well as transformation of hUCB-MCs into micro-glial and endothelial lines in ALS spinal cords. In addition to producing new nerve cells, providing degenerated areas with trophic factors and neo-vascularisation might prevent and even reverse progressive loss of moto-neurons and skeletal muscle paralysis.

• OSTI ID: 22416727
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 4 Vol. 451; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
BLOOD CELLS
DRUGS
GENES
HUMAN POPULATIONS
IN VIVO
MICE
MUSCLES
NERVE CELLS
SPINAL CORD
STEM CELLS
SYMPTOMS
THERAPY
TRANSCRIPTION FACTORS
TRANSPLANTS