Changes in seed water status as characterized by NMR in developing soybean seed grown under moisture stress conditions

Journal Article · Fri Feb 21 04:00:00 EST 2014 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2014.01.091· OSTI ID:22416277

Singh, Ravender; Verma, A. P.S.; Joshi, D. K.; Singh, Sheoraj

Highlights: • In developing soybean seeds, moisture stress resulted in more proportion of water to bound state. • These changes are further corroborated by concomitant changes in seed metabolites. • Thus there exists a moisture stress and development stage dependence of seed tissue water status. - Abstract: Changes in water status of developing seeds of Soybean (Glycine max L. Merrill.) grown under different moisture stress conditions were characterized by proton nuclear magnetic resonance (NMR)- spin–spin relaxation time (T{sub 2}). A comparison of the seed development characteristics, composition and physical properties indicated that, characteristics like seed weight, seed number/ear, rate of seed filling increased with development stages but decreased with moisture stress conditions. The NMR- spin–spin relaxation (T{sub 2}) component like bound water increased with seed maturation (40–50%) but decreased with moisture stress conditions (30–40%). The changes in seed water status to increasing levels of moisture stress and seed maturity indicates that moisture stress resulted in more proportion of water to bound state and intermediate state and less proportion of water in free-state. These changes are further corroborated by significant changes in protein and starch contents in seeds under high moisture stress treatments. Thus seed water status during its development is not only affected by development processes but also by moisture stress conditions. This study strongly indicated a clear moisture stress and development stage dependence of seed tissue water status in developing soybean seeds.

• OSTI ID: 22416277
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 4 Vol. 444; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
BOUND STATE
COMPARATIVE EVALUATIONS
GLYCINE
HYDROGEN 1
INTERMEDIATE STATE
MATURATION
METABOLITES
MOISTURE
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
PHYSICAL PROPERTIES
PLANT TISSUES
PROTEINS
RELAXATION TIME
SOYBEANS
SPIN
STARCH
STRESSES
WATER