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Title: Nonlinear photoacoustic spectroscopy of hemoglobin

Abstract

As light intensity increases in photoacoustic imaging, the saturation of optical absorption and the temperature dependence of the thermal expansion coefficient result in a measurable nonlinear dependence of the photoacoustic (PA) signal on the excitation pulse fluence. Here, under controlled conditions, we investigate the intensity-dependent photoacoustic signals from oxygenated and deoxygenated hemoglobin at varied optical wavelengths and molecular concentrations. The wavelength and concentration dependencies of the nonlinear PA spectrum are found to be significantly greater in oxygenated hemoglobin than in deoxygenated hemoglobin. These effects are further influenced by the hemoglobin concentration. These nonlinear phenomena provide insights into applications of photoacoustics, such as measurements of average inter-molecular distances on a nm scale or with a tuned selection of wavelengths, a more accurate quantitative PA tomography.

Authors:
; ; ; ;  [1]
  1. Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, One Brookings Drive, St. Louis, Missouri 63130 (United States)
Publication Date:
OSTI Identifier:
22402479
Resource Type:
Journal Article
Journal Name:
Applied Physics Letters
Additional Journal Information:
Journal Volume: 106; Journal Issue: 20; Other Information: (c) 2015 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0003-6951
Country of Publication:
United States
Language:
English
Subject:
71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS; CONCENTRATION RATIO; HEMOGLOBIN; NONLINEAR PROBLEMS; PHOTOACOUSTIC SPECTROSCOPY; PULSES; SIGNALS; TEMPERATURE DEPENDENCE; THERMAL EXPANSION; TOMOGRAPHY

Citation Formats

Danielli, Amos, Maslov, Konstantin, Favazza, Christopher P., Xia, Jun, and Wang, Lihong V., E-mail: LHWANG@WUSTL.EDU. Nonlinear photoacoustic spectroscopy of hemoglobin. United States: N. p., 2015. Web. doi:10.1063/1.4921474.
Danielli, Amos, Maslov, Konstantin, Favazza, Christopher P., Xia, Jun, & Wang, Lihong V., E-mail: LHWANG@WUSTL.EDU. Nonlinear photoacoustic spectroscopy of hemoglobin. United States. doi:10.1063/1.4921474.
Danielli, Amos, Maslov, Konstantin, Favazza, Christopher P., Xia, Jun, and Wang, Lihong V., E-mail: LHWANG@WUSTL.EDU. Mon . "Nonlinear photoacoustic spectroscopy of hemoglobin". United States. doi:10.1063/1.4921474.
@article{osti_22402479,
title = {Nonlinear photoacoustic spectroscopy of hemoglobin},
author = {Danielli, Amos and Maslov, Konstantin and Favazza, Christopher P. and Xia, Jun and Wang, Lihong V., E-mail: LHWANG@WUSTL.EDU},
abstractNote = {As light intensity increases in photoacoustic imaging, the saturation of optical absorption and the temperature dependence of the thermal expansion coefficient result in a measurable nonlinear dependence of the photoacoustic (PA) signal on the excitation pulse fluence. Here, under controlled conditions, we investigate the intensity-dependent photoacoustic signals from oxygenated and deoxygenated hemoglobin at varied optical wavelengths and molecular concentrations. The wavelength and concentration dependencies of the nonlinear PA spectrum are found to be significantly greater in oxygenated hemoglobin than in deoxygenated hemoglobin. These effects are further influenced by the hemoglobin concentration. These nonlinear phenomena provide insights into applications of photoacoustics, such as measurements of average inter-molecular distances on a nm scale or with a tuned selection of wavelengths, a more accurate quantitative PA tomography.},
doi = {10.1063/1.4921474},
journal = {Applied Physics Letters},
issn = {0003-6951},
number = 20,
volume = 106,
place = {United States},
year = {2015},
month = {5}
}