Crystallization and preliminary crystallographic analysis of the second GAF domain of DevS from Mycobacterium smegmatis

Journal Article · Tue Apr 01 00:00:00 EDT 2008 · Acta Crystallographica. Section F

DOI:https://doi.org/10.1107/S1744309108005186· OSTI ID:22360520

Cho, Ha Yeon; Cho, Hyo Je ^[1]; Kim, Young Min ^[2]; Oh, Jeong Il ^[3]

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1. School of Life Science and Biotechnology, Kyungpook National University, Daegu 702-701 (Korea, Republic of)
2. Department of Biology, Yonsei University, Seoul 120-749 (Korea, Republic of)
3. Department of Microbiology, Pusan National University, Pusan 609-735 (Korea, Republic of)

The second GAF domain of the hypoxia-sensing DevS from M. smegmatis has been crystallized. The crystal belonged to space group P6{sub 1} and diffracted to 2.0 Å resolution. Mycobacterium tuberculosis is known to transform into the nonreplicating persistence state under the influence of hypoxia or nitric oxide. DevS-DevR is a two-component regulatory system that mediates the genetic response for the transformation. DevS is a histidine kinase that contains two GAF domains for sensing hypoxia or nitric oxide. The second GAF from M. smegmatis DevS was crystallized using the sitting-drop vapour-diffusion method in the presence of sodium citrate and 2-propanol as precipitants. X-ray diffraction data were collected from crystals containing selenomethionine to a maximum resolution of 2.0 Å on a synchrotron beamline. The crystals belong to the hexagonal space group P6{sub 1}. The asymmetric unit contains one molecule, corresponding to a packing density of 2.5 Å{sup 3} Da{sup −1}. The selenium substructure was determined by the single anomalous dispersion method and structure refinement is in progress.

• OSTI ID: 22360520
• Journal Information: Acta Crystallographica. Section F, Vol. 64, Issue Pt 4; Other Information: PMCID: PMC2374252; PMID: 18391425; PUBLISHER-ID: ll5144; OAI: oai:pubmedcentral.nih.gov:2374252; Copyright (c) International Union of Crystallography 2008; Country of input: International Atomic Energy Agency (IAEA); ISSN 1744-3091
• Country of Publication: United Kingdom
• Language: English

Related Subjects

75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
CITRATES
CRYSTALLIZATION
CRYSTALS
DENSITY
DIFFUSION
HISTIDINE
MOLECULES
NITRIC OXIDE
PROPANOLS
SELENIUM
SODIUM COMPOUNDS
SPACE GROUPS
STOWING
X-RAY DIFFRACTION