Crystallization and preliminary crystallographic analysis of the second GAF domain of DevS from Mycobacterium smegmatis
Journal Article
·
· Acta Crystallographica. Section F
- School of Life Science and Biotechnology, Kyungpook National University, Daegu 702-701 (Korea, Republic of)
- Department of Biology, Yonsei University, Seoul 120-749 (Korea, Republic of)
- Department of Microbiology, Pusan National University, Pusan 609-735 (Korea, Republic of)
The second GAF domain of the hypoxia-sensing DevS from M. smegmatis has been crystallized. The crystal belonged to space group P6{sub 1} and diffracted to 2.0 Å resolution. Mycobacterium tuberculosis is known to transform into the nonreplicating persistence state under the influence of hypoxia or nitric oxide. DevS-DevR is a two-component regulatory system that mediates the genetic response for the transformation. DevS is a histidine kinase that contains two GAF domains for sensing hypoxia or nitric oxide. The second GAF from M. smegmatis DevS was crystallized using the sitting-drop vapour-diffusion method in the presence of sodium citrate and 2-propanol as precipitants. X-ray diffraction data were collected from crystals containing selenomethionine to a maximum resolution of 2.0 Å on a synchrotron beamline. The crystals belong to the hexagonal space group P6{sub 1}. The asymmetric unit contains one molecule, corresponding to a packing density of 2.5 Å{sup 3} Da{sup −1}. The selenium substructure was determined by the single anomalous dispersion method and structure refinement is in progress.
- OSTI ID:
- 22360520
- Journal Information:
- Acta Crystallographica. Section F, Journal Name: Acta Crystallographica. Section F Journal Issue: Pt 4 Vol. 64; ISSN ACSFCL; ISSN 1744-3091
- Country of Publication:
- United Kingdom
- Language:
- English
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