Crystallization and preliminary X-ray diffraction analysis of recombinant hepatitis E virus-like particle

Journal Article · Tue Apr 01 00:00:00 EDT 2008 · Acta Crystallographica. Section F

DOI:https://doi.org/10.1107/S1744309108007197· OSTI ID:22360511

Wang, Che-Yen ^[1]; Miyazaki, Naoyuki ^[1]; Yamashita, Tetsuo ^[2]; Higashiura, Akifumi; Nakagawa, Atsushi ^[2]; Li, Tian-Cheng; Takeda, Naokazu ^[3]; Xing, Li ^[1]; Hjalmarsson, Erik; Friberg, Claes ^[4]; Liou, Der-Ming ^[5]; Sung, Yen-Jen ^[5]; Tsukihara, Tomitake ^[2]; Matsuura, Yoshiharu ^[6]; Miyamura, Tatsuo ^[3]

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1. Molecular and Cellular Biology, University of California, Davis, CA 95616 (United States)
2. Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871 (Japan)
3. Department of Virology II, National Institute of Infectious Diseases, Tokyo (Japan)
4. Crystal Research AB, 22370 Lund (Sweden)
5. Institute of Public Health, National Yang-Ming University, 112 Taipei,Taiwan (China)
6. Institute for Microbial Diseases, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871 (Japan)

A recombinant virus-like particle that is a potential oral hepatitis E vaccine was crystallized. Diffraction data were collected to 8.3 Å resolution and the X-ray structure was phased with the aid of a low-resolution density map determined using cryo-electron microscopy data. Hepatitis E virus (HEV) accounts for the majority of enterically transmitted hepatitis infections worldwide. Currently, there is no specific treatment for or vaccine against HEV. The major structural protein is derived from open reading frame (ORF) 2 of the viral genome. A potential oral vaccine is provided by the virus-like particles formed by a protein construct of partial ORF3 protein (residue 70–123) fused to the N-terminus of the ORF2 protein (residues 112–608). Single crystals obtained by the hanging-drop vapour-diffusion method at 293 K diffract X-rays to 8.3 Å resolution. The crystals belong to space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 337, b = 343, c = 346 Å, α = β = γ = 90°, and contain one particle per asymmetric unit.

• OSTI ID: 22360511
• Journal Information: Acta Crystallographica. Section F, Vol. 64, Issue Pt 4; Other Information: PMCID: PMC2374242; PMID: 18391436; PUBLISHER-ID: en5297; OAI: oai:pubmedcentral.nih.gov:2374242; Copyright (c) International Union of Crystallography 2008; Country of input: International Atomic Energy Agency (IAEA); ISSN 1744-3091
• Country of Publication: United Kingdom
• Language: English

Related Subjects

75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
CRYSTALLIZATION
DENSITY
DIFFUSION
ELECTRON MICROSCOPY
MONOCRYSTALS
POTENTIALS
PROTEINS
RESOLUTION
SPACE GROUPS
X-RAY DIFFRACTION