Crystallization and preliminary crystallographic analysis of a plant type III polyketide synthase that produces benzalacetone

Journal Article · Tue Apr 01 04:00:00 EDT 2008 · Acta Crystallographica. Section F

DOI:https://doi.org/10.1107/S1744309108006775· OSTI ID:22360508

Morita, Hiroyuki; Tanio, Michikazu ^[1]; Kondo, Shin; Kato, Ryohei ^[2]; Wanibuchi, Kiyofumi; Noguchi, Hiroshi ^[3]; Sugio, Shigetoshi ^[2]; Abe, Ikuro ^[3]; Kohno, Toshiyuki ^[1]

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1. Mitsubishi Kagaku Institute of Life Sciences (MITILS), 11 Minamiooya, Machida, Tokyo 194-8511 (Japan)
2. Innovation Center Yokohama, Mitsubishi Chemical Corporation, 1000 Kamoshida, Aoba, Yokohama, Kanagawa 227-8502 (Japan)
3. School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka 422-8526 (Japan)

Benzalacetone synthase from R. palmatum has been overexpressed in E. coli, purified and crystallized. Diffraction data have been collected to a resolution of 1.8 Å. Benzalacetone synthase (BAS) from Rheum palmatum is a plant-specific type III polyketide synthase that catalyzes the one-step decarboxylative condensation of 4-coumaroyl-CoA with malonyl-CoA to produce the diketide 4-(4-hydroxyphenyl)-but-3-en-2-one. Recombinant BAS expressed in Escherichia coli was crystallized by the sitting-drop vapour-diffusion method. The crystals belong to space group P2{sub 1}, with unit-cell parameters a = 54.6, b = 89.6, c = 81.1 Å, α = γ = 90.0, β = 100.5°. Diffraction data were collected to 1.8 Å resolution using synchrotron radiation at BL24XU of SPring-8.

• OSTI ID: 22360508
• Journal Information: Acta Crystallographica. Section F, Journal Name: Acta Crystallographica. Section F Journal Issue: Pt 4 Vol. 64; ISSN ACSFCL; ISSN 1744-3091
• Country of Publication: United Kingdom
• Language: English

Related Subjects

75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
CRYSTALLIZATION
CRYSTALS
DIFFRACTION
DIFFUSION
ESCHERICHIA COLI
RESOLUTION
SPACE GROUPS
SYNCHROTRON RADIATION