Crystallization and preliminary X-ray diffraction analysis of a glutathione S-transferase from Xylella fastidiosa

Journal Article · Fri Feb 01 04:00:00 EST 2008 · Acta Crystallographica. Section F

DOI:https://doi.org/10.1107/S174430910706825X· OSTI ID:22360500

Garcia, Wanius ^[1]; Travensolo, Regiane F. ^[2]; Rodrigues, Nathalia C.; Muniz, João R. C. ^[1]; Caruso, Célia S. ^[2]; Lemos, Eliana G. M. ^[3]; Araujo, Ana Paula U. ^[1]; Carrilho, Emanuel ^[2]

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1. Laboratório de Biofísica Molecular ‘Sérgio Mascarenhas’, Instituto de Física de São Carlos, Universidade de São Paulo (USP), São Carlos (Brazil)
2. Grupo de Bioanalítica, Microfabricação e Separações, Instituto de Química de São Carlos, Universidade de São Paulo (USP), São Carlos (Brazil)
3. Laboratório de Bioquímica de Microrganismos e de Plantas, Departamento de Tecnologia, UNESP, Jaboticabal (Brazil)

Glutathione S-transferase from X. fastidiosa (xfGST) has been overexpressed in E. coli, purified and crystallized. Diffraction data were collected to 2.23 Å. Glutathione S-transferases (GSTs) form a group of multifunctional isoenzymes that catalyze the glutathione-dependent conjugation and reduction reactions involved in the cellular detoxification of xenobiotic and endobiotic compounds. GST from Xylella fastidiosa (xfGST) was overexpressed in Escherichia coli and purified by conventional affinity chromatography. In this study, the crystallization and preliminary X-ray analysis of xfGST is described. The purified protein was crystallized by the vapour-diffusion method, producing crystals that belonged to the triclinic space group P1. The unit-cell parameters were a = 47.73, b = 87.73, c = 90.74 Å, α = 63.45, β = 80.66, γ = 94.55°. xfGST crystals diffracted to 2.23 Å resolution on a rotating-anode X-ray source.

• OSTI ID: 22360500
• Journal Information: Acta Crystallographica. Section F, Journal Name: Acta Crystallographica. Section F Journal Issue: Pt 2 Vol. 64; ISSN ACSFCL; ISSN 1744-3091
• Country of Publication: United Kingdom
• Language: English

Related Subjects

75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
AFFINITY
ANODES
CRYSTALLIZATION
CRYSTALS
DIFFUSION
ESCHERICHIA COLI
REDUCTION
RESOLUTION
SPACE GROUPS
X-RAY DIFFRACTION
X-RAY SOURCES