Crystallization and preliminary X-ray study of native and selenomethionyl β-1,4-mannanase AaManA from Alicyclobacillus acidocaldarius Tc-12-31
- State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101 (China)
- Graduate School, Chinese Academy of Sciences, Beijing 100049 (China)
A. acidocaldarius Tc-12-31 β-1,4-mannanase was expressed in E. coli and purified. Crystallization and preliminarily X-ray crystallographic studies were performed for both the native mannanase and its selenomethionyl derivative. AaManA, a β-1,4-mannanase from the thermoacidophile Alicyclobacillus acidocaldarius Tc-12-31, was expressed in Escherichia coli and purified in a form suitable for X-ray crystallographic analysis. Crystals were obtained using the hanging-drop vapour-diffusion method at 291 K using ammonium dihydrogen phosphate as a precipitant. Data were collected from native mannanase and from a selenomethionyl derivative to 1.90 and 1.99 Å, respectively, at 100 K. The native crystal belongs to the orthorhombic space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 44.34, b = 75.55, c = 88.02 Å. The derivative crystal belonged to the same space group as native AaManA, with unit-cell parameters a = 44.55, b = 75.70, c = 92.66 Å.
- OSTI ID:
- 22360482
- Journal Information:
- Acta Crystallographica. Section F, Journal Name: Acta Crystallographica. Section F Journal Issue: Pt 3 Vol. 64; ISSN ACSFCL; ISSN 1744-3091
- Country of Publication:
- United Kingdom
- Language:
- English
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