Purification, identification and preliminary crystallographic studies of Pru du amandin, an allergenic protein from Prunus dulcis
The purification, identification, crystallization and preliminary crystallographic studies of an allergy-related protein, Pru du amandin, from P. dulcis nuts are reported. Food allergies appear to be one of the foremost causes of hypersensitivity reactions. Nut allergies account for most food allergies and are often permanent. The 360 kDa hexameric protein Pru du amandin, a known allergen, was purified from almonds (Prunus dulcis) by ammonium sulfate fractionation and ion-exchange chromatography. The protein was identified by a BLAST homology search against the nonredundant sequence database. Pru du amandin belongs to the 11S legumin family of seed storage proteins characterized by the presence of a cupin motif. Crystals were obtained by the hanging-drop vapour-diffusion method. The crystals belong to space group P4{sub 1} (or P4{sub 3}), with unit-cell parameters a = b = 150.7, c = 164.9 Å.
- OSTI ID:
- 22360466
- Journal Information:
- Acta Crystallographica. Section F, Vol. 64, Issue Pt 1; Other Information: PMCID: PMC2373995; PMID: 18097098; PUBLISHER-ID: hc5040; OAI: oai:pubmedcentral.nih.gov:2373995; Copyright (c) International Union of Crystallography 2008; Country of input: International Atomic Energy Agency (IAEA); ISSN 1744-3091
- Country of Publication:
- United Kingdom
- Language:
- English
Similar Records
Crystal Structure of Prunin-1, a Major Component of the Almond (Prunus dulcis) Allergen Amandin
Purification, crystallization and initial crystallographic characterization of brazil-nut allergen Ber e 2