Crystallization and preliminary X-ray analysis of an arabinoxylan arabinofuranohydrolase from Bacillus subtilis

Journal Article · Wed Aug 01 04:00:00 EDT 2007 · Acta Crystallographica. Section F

DOI:https://doi.org/10.1107/S1744309107033702· OSTI ID:22360387

Vandermarliere, Elien ^[1]; Bourgois, Tine M.; Van Campenhout, Steven ^[2]; Strelkov, Sergei V. ^[1]; Volckaert, Guido ^[2]; Delcour, Jan A.; Courtin, Christophe M. ^[3]; Rabijns, Anja ^[1]

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1. Laboratory for Biocrystallography, Katholieke Universiteit Leuven, Herestraat 49, O&N II bus 822, 3000 Leuven (Belgium)
2. Laboratory of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, 3001 Leuven (Belgium)
3. Laboratory of Food Chemistry and Biochemistry, Department of Molecular and Microbial Systems, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20 bus 2463, 3001 Leuven (Belgium)

The crystallization and preliminary X-ray analysis of the family 43 glycoside hydrolase arabinoxylan arabinofuranohydrolase from B. subtilis soaked with xylotriose is described in order to gain insight in the way the enzyme binds its substrates. Arabinoxylan arabinofuranohydrolases (AXH) are α-l-arabinofuranosidases (EC 3.2.1.55) that specifically hydrolyse the glycosidic bond between arabinofuranosyl substituents and xylopyranosyl residues from arabinoxylan, hence their name. In this study, the crystallization and preliminary X-ray analysis of the AXH from Bacillus subtilis, a glycoside hydrolase belonging to family 43, is described. Purified recombinant AXH crystallized in the orthorhombic space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 68.7, b = 73.7, c = 106.5 Å. X-ray diffraction data were collected to a resolution of 1.55 Å.

• OSTI ID: 22360387
• Journal Information: Acta Crystallographica. Section F, Journal Name: Acta Crystallographica. Section F Journal Issue: Pt 8 Vol. 63; ISSN ACSFCL; ISSN 1744-3091
• Country of Publication: United Kingdom
• Language: English

Related Subjects

75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
CRYSTALLIZATION
RESOLUTION
SPACE GROUPS
SUBSTRATES
X-RAY DIFFRACTION