Expression, crystallization and preliminary X-ray diffraction studies of recombinant Clostridium perfringens β2-toxin
- Department of Veterinary and Biomedical Science, The Pennsylvania State University (United States)
- Macromolecular X-ray Crystallography Facility, The Pennsylvania State University (United States)
- Argonne National Laboratory (United States)
The cloning, expression, purification and crystallization of recombinant Clostridium perfringens β2-toxin is described. The crystals diffracted to 2.9 Å resolution. Clostridium perfringens is a Gram-positive sporulating anaerobic bacterium that is responsible for a wide spectrum of diseases in animals, birds and humans. The virulence of C. perfringens is associated with the production of several enterotoxins and exotoxins. β2-toxin is a 28 kDa exotoxin produced by C. perfringens. It is implicated in necrotic enteritis in animals and humans, a disease characterized by a sudden acute onset with lethal hemorrhagic mucosal ulceration. The recombinant expression, purification and crystallization of β2-toxin using the batch-under-oil technique are reported here. Native X-ray diffraction data were obtained to 2.9 Å resolution on a synchrotron beamline at the F2 station at Cornell High Energy Synchrotron Source (CHESS) using an ADSC Quantum-210 CCD detector. The crystals belong to space group R3, with a dimer in the asymmetric unit; the unit-cell parameters are a = b = 103.71, c = 193.48 Å, α = β = 90, γ = 120° using the hexagonal axis setting. A self-rotation function shows that the two molecules are related by a noncrystallographic twofold axis with polar angles ω = 90.0, ϕ = 210.3°.
- OSTI ID:
- 22360350
- Journal Information:
- Acta Crystallographica. Section F, Vol. 63, Issue Pt 6; Other Information: PMCID: PMC2335086; PMID: 17554168; PUBLISHER-ID: pu5178; OAI: oai:pubmedcentral.nih.gov:2335086; Copyright (c) International Union of Crystallography 2007; Country of input: International Atomic Energy Agency (IAEA); ISSN 1744-3091
- Country of Publication:
- United Kingdom
- Language:
- English
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