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Title: Improved crystallization of the coxsackievirus B3 RNA-dependent RNA polymerase

Abstract

The first crystal of a coxsackievirus RNA-dependent RNA polymerase is reported. The Picornaviridae virus family contains a large number of human pathogens such as poliovirus, hepatitis A virus and rhinoviruses. Amongst the viruses belonging to the genus Enterovirus, several serotypes of coxsackievirus coexist for which neither vaccine nor therapy is available. Coxsackievirus B3 is involved in the development of acute myocarditis and dilated cardiomyopathy and is thought to be an important cause of sudden death in young adults. Here, the first crystal of a coxsackievirus RNA-dependent RNA polymerase is reported. Standard crystallization methods yielded crystals that were poorly suited to X-ray diffraction studies, with one axis being completely disordered. Crystallization was improved by testing crystallization solutions from commercial screens as additives. This approach yielded crystals that diffracted to 2.1 Å resolution and that were suitable for structure determination.

Authors:
; ; ; ; ; ; ; ; ; ; ; ;  [1]
  1. Centre National de la Recherche Scientifique and Universités d’Aix-Marseille I et II, UMR 6098, Architecture et Fonction des Macromolécules Biologiques, Ecole Supérieure d’Ingénieurs de Luminy-Case 925, 163 Avenue de Luminy, 13288 Marseille CEDEX 9 (France)
Publication Date:
OSTI Identifier:
22360343
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallographica. Section F; Journal Volume: 63; Journal Issue: Pt 6; Other Information: PMCID: PMC2335076; PMID: 17554171; PUBLISHER-ID: en5233; OAI: oai:pubmedcentral.nih.gov:2335076; Copyright (c) International Union of Crystallography 2007; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United Kingdom
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CRYSTALLIZATION; CRYSTALS; DIFFRACTION; MATHEMATICAL SOLUTIONS; RESOLUTION; SOLUTIONS; TESTING

Citation Formats

Jabafi, Ilham, Selisko, Barbara, Coutard, Bruno, De Palma, Armando M., Neyts, Johan, Egloff, Marie-Pierre, Grisel, Sacha, Dalle, Karen, Campanacci, Valerie, Spinelli, Silvia, Cambillau, Christian, Canard, Bruno, and Gruez, Arnaud, E-mail: arnaud.gruez@maem.uhp-nancy.fr. Improved crystallization of the coxsackievirus B3 RNA-dependent RNA polymerase. United Kingdom: N. p., 2007. Web. doi:10.1107/S1744309107020416.
Jabafi, Ilham, Selisko, Barbara, Coutard, Bruno, De Palma, Armando M., Neyts, Johan, Egloff, Marie-Pierre, Grisel, Sacha, Dalle, Karen, Campanacci, Valerie, Spinelli, Silvia, Cambillau, Christian, Canard, Bruno, & Gruez, Arnaud, E-mail: arnaud.gruez@maem.uhp-nancy.fr. Improved crystallization of the coxsackievirus B3 RNA-dependent RNA polymerase. United Kingdom. doi:10.1107/S1744309107020416.
Jabafi, Ilham, Selisko, Barbara, Coutard, Bruno, De Palma, Armando M., Neyts, Johan, Egloff, Marie-Pierre, Grisel, Sacha, Dalle, Karen, Campanacci, Valerie, Spinelli, Silvia, Cambillau, Christian, Canard, Bruno, and Gruez, Arnaud, E-mail: arnaud.gruez@maem.uhp-nancy.fr. Fri . "Improved crystallization of the coxsackievirus B3 RNA-dependent RNA polymerase". United Kingdom. doi:10.1107/S1744309107020416.
@article{osti_22360343,
title = {Improved crystallization of the coxsackievirus B3 RNA-dependent RNA polymerase},
author = {Jabafi, Ilham and Selisko, Barbara and Coutard, Bruno and De Palma, Armando M. and Neyts, Johan and Egloff, Marie-Pierre and Grisel, Sacha and Dalle, Karen and Campanacci, Valerie and Spinelli, Silvia and Cambillau, Christian and Canard, Bruno and Gruez, Arnaud, E-mail: arnaud.gruez@maem.uhp-nancy.fr},
abstractNote = {The first crystal of a coxsackievirus RNA-dependent RNA polymerase is reported. The Picornaviridae virus family contains a large number of human pathogens such as poliovirus, hepatitis A virus and rhinoviruses. Amongst the viruses belonging to the genus Enterovirus, several serotypes of coxsackievirus coexist for which neither vaccine nor therapy is available. Coxsackievirus B3 is involved in the development of acute myocarditis and dilated cardiomyopathy and is thought to be an important cause of sudden death in young adults. Here, the first crystal of a coxsackievirus RNA-dependent RNA polymerase is reported. Standard crystallization methods yielded crystals that were poorly suited to X-ray diffraction studies, with one axis being completely disordered. Crystallization was improved by testing crystallization solutions from commercial screens as additives. This approach yielded crystals that diffracted to 2.1 Å resolution and that were suitable for structure determination.},
doi = {10.1107/S1744309107020416},
journal = {Acta Crystallographica. Section F},
number = Pt 6,
volume = 63,
place = {United Kingdom},
year = {Fri Jun 01 00:00:00 EDT 2007},
month = {Fri Jun 01 00:00:00 EDT 2007}
}