Expression, crystallization and preliminary crystallographic data analysis of filamin A repeats 14–16
Journal Article
·
· Acta Crystallographica. Section F
- Department of Medical Biochemistry and Microbiology, Uppsala University (Sweden)
The crystallization and crystallographic data analysis of filamin repeats 14–16 are reported. Human filamin A is a 280 kDa protein involved in actin-filament cross-linking. It is structurally divided into an actin-binding headpiece (ABD) and a rod domain containing 24 immunoglobulin-like (Ig) repeats. A fragment of human filamin A (Ig repeats 14–16) was cloned and expressed in Escherichia coli and the purified protein was crystallized in 1.6 M ammonium sulfate, 2% PEG 1000 and 100 mM HEPES pH 7.5. The crystals diffracted to 1.95 Å and belong to space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 50.63, b = 52.10, c = 98.46 Å, α = β = γ = 90°.
- OSTI ID:
- 22360291
- Journal Information:
- Acta Crystallographica. Section F, Journal Name: Acta Crystallographica. Section F Journal Issue: Pt 4 Vol. 63; ISSN ACSFCL; ISSN 1744-3091
- Country of Publication:
- United Kingdom
- Language:
- English
Similar Records
Expression, purification and preliminary crystallographic analysis of recombinant human small glutamine-rich tetratricopeptide-repeat protein
Actin-binding protein (ABP-280) filamin gene (FLN) maps telomeric to the color vision locus (R/GCP) and centromeric to G6PD in Xq28
Filamin A negatively regulates the transcriptional activity of p73{alpha} in the cytoplasm
Journal Article
·
Tue Jul 01 00:00:00 EDT 2008
· Acta Crystallographica. Section F
·
OSTI ID:22360592
Actin-binding protein (ABP-280) filamin gene (FLN) maps telomeric to the color vision locus (R/GCP) and centromeric to G6PD in Xq28
Journal Article
·
Sun Aug 01 00:00:00 EDT 1993
· Genomics; (United States)
·
OSTI ID:7035439
Filamin A negatively regulates the transcriptional activity of p73{alpha} in the cytoplasm
Journal Article
·
Sat Nov 03 00:00:00 EDT 2007
· Biochemical and Biophysical Research Communications
·
OSTI ID:21032957