Expression, purification, crystallization and preliminary X-ray diffraction analysis of the VP8* sialic acid-binding domain of porcine rotavirus strain OSU
Journal Article
·
· Acta Crystallographica. Section F
- National Hepatobiliary and Enteric Surgery Research Center of The Ministry of Health, Xiangya Hospital, Central South University, Hunan Province (China)
- Biochemistry Laboratory, Institution of Biomedical Engineering, Central South University, Hunan Province (China)
Porcine rotavirus strain OSU VP8* domain has been expressed, purified and crystallized. X-ray diffraction data from different crystal forms of the VP8* domain have been collected to 2.65 and 2.2 Å resolution, respectively. The rotavirus outer capsid spike protein VP4 is utilized in the process of rotavirus attachment to and membrane penetration of host cells. VP4 is cleaved by trypsin into two domains: VP8* and VP5*. The VP8* domain is implicated in initial interaction with sialic acid-containing cell-surface carbohydrates and triggers subsequent virus invasion. The VP8* domain from porcine OSU rotavirus was cloned and expressed in Escherichia coli. Different crystal forms (orthorhombic P2{sub 1}2{sub 1}2{sub 1} and tetragonal P4{sub 1}2{sub 1}2) were harvested from two distinct crystallization conditions. Diffraction data have been collected to 2.65 and 2.2 Å resolution and the VP8*{sub 65–224} structure was determined by molecular replacement.
- OSTI ID:
- 22360267
- Journal Information:
- Acta Crystallographica. Section F, Journal Name: Acta Crystallographica. Section F Journal Issue: Pt 2 Vol. 63; ISSN ACSFCL; ISSN 1744-3091
- Country of Publication:
- United Kingdom
- Language:
- English
Similar Records
Crystallization and preliminary X-ray diffraction analysis of the sialic acid-binding domain (VP8*) of porcine rotavirus strain CRW-8
Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the VP8* carbohydrate-binding protein of the human rotavirus strain Wa
Novel fold of rotavirus glycan-binding domain predicted by AlphaFold2 and determined by X-ray crystallography
Journal Article
·
Wed Jun 01 00:00:00 EDT 2005
· Acta Crystallographica. Section F
·
OSTI ID:22356019
Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the VP8* carbohydrate-binding protein of the human rotavirus strain Wa
Journal Article
·
Mon Oct 31 23:00:00 EST 2005
· Acta Crystallographica. Section F
·
OSTI ID:22356180
Novel fold of rotavirus glycan-binding domain predicted by AlphaFold2 and determined by X-ray crystallography
Journal Article
·
Wed May 04 20:00:00 EDT 2022
· Communications Biology
·
OSTI ID:1880816