Crystallographic characterization of the radixin FERM domain bound to the cytoplasmic tails of adhesion molecules CD43 and PSGL-1
Journal Article
·
· Acta Crystallographica. Section F
- Structural Biology Laboratory, Nara Institute of Science and Technology, Keihanna Science City, Nara 630-0192 (Japan)
The radixin FERM domain has been crystallized in complex with CD43 and PSGL-1 peptides. Diffraction data sets were collected from the complexes to 2.9 and 2.8 Å resolution, respectively. Radixin is a member of the ERM proteins that cross-link plasma membranes and actin filaments. The FERM domains located in the N-terminal regions of ERM proteins are responsible for membrane association through direct interaction with the cytoplasmic tails of integral membrane proteins. Here, crystals of the radixin FERM domain bound to the cytoplasmic peptides of two adhesion molecules, CD43 and PSGL-1, have been obtained. Crystals of the radixin FERM domain bound to CD43 belong to space group P4{sub 3}22, with unit-cell parameters a = b = 68.72, c = 201.39 Å, and contain one complex in the crystallographic asymmetric unit. Crystals of the radixin FERM domain bound to PSGL-1 belong to space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 80.74, b = 85.73, c = 117.75 Å, and contain two complexes in the crystallographic asymmetric unit. Intensity data sets were collected to a resolution of 2.9 Å for the FERM–CD43 complex and 2.8 Å for the FERM–PSGL-1 complex.
- OSTI ID:
- 22360249
- Journal Information:
- Acta Crystallographica. Section F, Journal Name: Acta Crystallographica. Section F Journal Issue: Pt 1 Vol. 63; ISSN ACSFCL; ISSN 1744-3091
- Country of Publication:
- United Kingdom
- Language:
- English
Similar Records
Crystallographic characterization of the radixin FERM domain bound to the cytoplasmic tail of adhesion molecule CD44
Structure of dimerized radixin FERM domain suggests a novel masking motif in C-terminal residues 295–304
Unfurling of the band 4.1, ezrin, radixin, moesin (FERM) domain of the merlin tumor suppressor
Journal Article
·
Mon Oct 01 00:00:00 EDT 2007
· Acta Crystallographica. Section F
·
OSTI ID:22360404
Structure of dimerized radixin FERM domain suggests a novel masking motif in C-terminal residues 295–304
Journal Article
·
Fri Mar 31 23:00:00 EST 2006
· Acta Crystallographica. Section F
·
OSTI ID:22356351
Unfurling of the band 4.1, ezrin, radixin, moesin (FERM) domain of the merlin tumor suppressor
Journal Article
·
Thu Oct 02 00:00:00 EDT 2014
· Protein Sci.
·
OSTI ID:1031383