Crystallization and preliminary crystallographic analysis of molybdenum-cofactor biosynthesis protein C from Thermus thermophilus

Journal Article · Sun Dec 31 23:00:00 EST 2006 · Acta Crystallographica. Section F

DOI:https://doi.org/10.1107/S1744309106052560· OSTI ID:22360245

Kanaujia, Shankar Prasad; Ranjani, Chellamuthu Vasuki ^[1]; Jeyakanthan, Jeyaraman ^[2]; Baba, Seiki ^[2]; Chen, Lirong; Liu, Zhi-Jie; Wang, Bi-Cheng ^[3]; Nishida, Masami; Ebihara, Akio; Shinkai, Akeo ^[2]; Kuramitsu, Seiki ^[2]; Shiro, Yoshitsugu ^[2]; Sekar, Kanagaraj ^[1]; Yokoyama, Shigeyuki ^[2]

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1. Bioinformatics Centre (Centre of Excellence in Structural Biology and Bio-computing), Indian Institute of Science, Bangalore 560 012 (India)
2. RIKEN SPring-8 Center, Harima Institute, 1-1-1 Kouto, Sayo, Hyogo 679-5148 (Japan)
3. Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602 (United States)

The molybdenum-cofactor biosynthesis protein C from T. thermophilus has been crystallized in two different space groups, P2{sub 1} and R32; the crystals diffracted to 1.9 and 1.75 Å resolution, respectively. The Gram-negative aerobic eubacterium Thermus thermophilus is an extremely important thermophilic microorganism that was originally isolated from a thermal vent environment in Japan. The molybdenum cofactor in this organism is considered to be an essential component required by enzymes that catalyze diverse key reactions in the global metabolism of carbon, nitrogen and sulfur. The molybdenum-cofactor biosynthesis protein C derived from T. thermophilus was crystallized in two different space groups. Crystals obtained using the first crystallization condition belong to the monoclinic space group P2{sub 1}, with unit-cell parameters a = 64.81, b = 109.84, c = 115.19 Å, β = 104.9°; the crystal diffracted to a resolution of 1.9 Å. The other crystal form belonged to space group R32, with unit-cell parameters a = b = 106.57, c = 59.25 Å, and diffracted to 1.75 Å resolution. Preliminary calculations reveal that the asymmetric unit contains 12 monomers and one monomer for the crystals belonging to space group P2{sub 1} and R32, respectively.

• OSTI ID: 22360245
• Journal Information: Acta Crystallographica. Section F, Journal Name: Acta Crystallographica. Section F Journal Issue: Pt 1 Vol. 63; ISSN ACSFCL; ISSN 1744-3091
• Country of Publication: United Kingdom
• Language: English

Related Subjects

75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
CARBON
CRYSTALLIZATION
CRYSTALS
ENVIRONMENT
IRON
MOLYBDENUM
MONOMERS
NITROGEN
RESOLUTION
SPACE GROUPS
SULFUR