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Title: Purification, crystallization and preliminary crystallographic characterization of the caspase-recruitment domain of human Nod1

Abstract

The caspase-recruitment domain of the cytosolic pathogen receptor Nod1 was crystallized. X-ray diffraction data were collected to 1.9 Å resolution. The caspase-recruitment domain (CARD) is known to play an important role in apoptosis and inflammation as an essential protein–protein interaction domain. The CARD of the cytosolic pathogen receptor Nod1 was overexpressed in Escherichia coli and purified by affinity chromatography and gel filtration. The purified CARD was crystallized at 277 K using the microseeding method. X-ray diffraction data were collected to 1.9 Å resolution. The crystals belong to space group P3{sub 1} or P3{sub 2}, with unit-cell parameters a = b = 79.1, c = 80.9 Å. Preliminary analysis indicates that there is one dimeric CARD molecule in the asymmetric unit.

Authors:
; ;  [1];  [2];  [1]
  1. Basic Science, Fox Chase Cancer Center, Philadelphia, PA 19111 (United States)
  2. Department of Clinical Laboratory Science, Shinheung College, Uijeongbu, Kyungki-Do 480-701 (Korea, Republic of)
Publication Date:
OSTI Identifier:
22360243
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallographica. Section F; Journal Volume: 63; Journal Issue: Pt 1; Other Information: PMCID: PMC2330101; PMID: 17183166; PUBLISHER-ID: bw5173; OAI: oai:pubmedcentral.nih.gov:2330101; Copyright (c) International Union of Crystallography 2007; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United Kingdom
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; AFFINITY; CRYSTALLIZATION; CRYSTALS; ESCHERICHIA COLI; GELS; INTERACTIONS; MOLECULES; RECEPTORS; RESOLUTION; SPACE GROUPS; X-RAY DIFFRACTION

Citation Formats

Srimathi, Thiagarajan, Robbins, Sheila L., Dubas, Rachel L., Seo, Jang-Hoon, and Park, Young Chul, E-mail: young.park@fccc.edu. Purification, crystallization and preliminary crystallographic characterization of the caspase-recruitment domain of human Nod1. United Kingdom: N. p., 2007. Web. doi:10.1107/S1744309106051955.
Srimathi, Thiagarajan, Robbins, Sheila L., Dubas, Rachel L., Seo, Jang-Hoon, & Park, Young Chul, E-mail: young.park@fccc.edu. Purification, crystallization and preliminary crystallographic characterization of the caspase-recruitment domain of human Nod1. United Kingdom. doi:10.1107/S1744309106051955.
Srimathi, Thiagarajan, Robbins, Sheila L., Dubas, Rachel L., Seo, Jang-Hoon, and Park, Young Chul, E-mail: young.park@fccc.edu. Mon . "Purification, crystallization and preliminary crystallographic characterization of the caspase-recruitment domain of human Nod1". United Kingdom. doi:10.1107/S1744309106051955.
@article{osti_22360243,
title = {Purification, crystallization and preliminary crystallographic characterization of the caspase-recruitment domain of human Nod1},
author = {Srimathi, Thiagarajan and Robbins, Sheila L. and Dubas, Rachel L. and Seo, Jang-Hoon and Park, Young Chul, E-mail: young.park@fccc.edu},
abstractNote = {The caspase-recruitment domain of the cytosolic pathogen receptor Nod1 was crystallized. X-ray diffraction data were collected to 1.9 Å resolution. The caspase-recruitment domain (CARD) is known to play an important role in apoptosis and inflammation as an essential protein–protein interaction domain. The CARD of the cytosolic pathogen receptor Nod1 was overexpressed in Escherichia coli and purified by affinity chromatography and gel filtration. The purified CARD was crystallized at 277 K using the microseeding method. X-ray diffraction data were collected to 1.9 Å resolution. The crystals belong to space group P3{sub 1} or P3{sub 2}, with unit-cell parameters a = b = 79.1, c = 80.9 Å. Preliminary analysis indicates that there is one dimeric CARD molecule in the asymmetric unit.},
doi = {10.1107/S1744309106051955},
journal = {Acta Crystallographica. Section F},
number = Pt 1,
volume = 63,
place = {United Kingdom},
year = {Mon Jan 01 00:00:00 EST 2007},
month = {Mon Jan 01 00:00:00 EST 2007}
}