Crystallization and preliminary X-ray diffraction analysis of protein 14 from Sulfolobus islandicus filamentous virus (SIFV)
- Architecture et Fonction des Macromolécules Biologiques, CNRS and Universités d’Aix-Marseille I et II, UMR 6098, Case 932, 163 Avenue de Luminy, 13288 Marseille CEDEX 9 (France)
- Danish Archaea Centre, Institute of Molecular Biology, Copenhagen University, Soelvgade 83H, DK1307 Copenhagen K (Denmark)
- Institut de Biochimie et de Biophysique Moléculaire et Cellulaire (CNRS-UMR 8619), Université Paris 11, Bâtiment 430, 91405 Orsay (France)
- Institut Pasteur, Unité de Biologie Moléculaire du Gène chez les Extrêmophiles, 25 Rue du Dr Roux, 75724 Paris CEDEX 15 (France)
Crystals of S. islandicus filamentous virus (SIFV) protein 14 have been grown at 293 K. Crystals belong to space group P6{sub 2}22 or P6{sub 4}22 and diffract to a resolution of 2.95 Å. A large-scale programme has been embarked upon aiming towards the structural determination of conserved proteins from viruses infecting hyperthermophilic archaea. Here, the crystallization of protein 14 from the archaeal virus SIFV is reported. This protein, which contains 111 residues (MW 13 465 Da), was cloned and expressed in Escherichia coli with an N-terminal His{sub 6} tag and purified to homogeneity. The tag was subsequently cleaved and the protein was crystallized using PEG 1000 or PEG 4000 as a precipitant. Large crystals were obtained of the native and the selenomethionine-labelled protein using sitting drops of 100–300 nl. Crystals belong to space group P6{sub 2}22 or P6{sub 4}22, with unit-cell parameters a = b = 68.1, c = 132.4 Å. Diffraction data were collected to a maximum acceptable resolution of 2.95 and 3.20 Å for the SeMet-labelled and native protein, respectively.
- OSTI ID:
- 22356434
- Journal Information:
- Acta Crystallographica. Section F, Vol. 62, Issue Pt 9; Other Information: PMCID: PMC2242861; PMID: 16946470; PUBLISHER-ID: bw5157; OAI: oai:pubmedcentral.nih.gov:2242861; Copyright (c) International Union of Crystallography 2006; Country of input: International Atomic Energy Agency (IAEA); ISSN 1744-3091
- Country of Publication:
- United Kingdom
- Language:
- English
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