Cloning, expression, purification, crystallization and preliminary X-ray analysis of peptidyl-tRNA hydrolase from Mycobacterium tuberculosis
Journal Article
·
· Acta Crystallographica. Section F
- Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560 012 (India)
- Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560 012 (India)
The peptidyl-tRNA hydrolase from M. tuberculosis has been crystallized in three closely related forms, two orthorhombic and one monoclinic, and X-ray diffraction data have been collected from them. Peptidyl-tRNA hydrolase catalyses the cleavage of the ester link between the peptide and the tRNA in peptidyl-tRNAs that, for various reasons, have dropped off the translating ribosomes. This enzyme from Mycobacterium tuberculosis has been crystallized in three related but distinct forms: P2{sub 1}2{sub 1}2{sub 1}, unit-cell parameters a = 36.30, b = 61.85, c = 73.97 Å, P2{sub 1}, a = 35.83, b = 73.79, c = 59.79 Å, β = 92.3°, and P2{sub 1}2{sub 1}2{sub 1}, a = 35.84, b = 57.06, c = 72.59 Å. X-ray data have been collected from all three forms.
- OSTI ID:
- 22356433
- Journal Information:
- Acta Crystallographica. Section F, Journal Name: Acta Crystallographica. Section F Journal Issue: Pt 9 Vol. 62; ISSN ACSFCL; ISSN 1744-3091
- Country of Publication:
- United Kingdom
- Language:
- English
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