Crystallization and preliminary X-ray analysis of the C-terminal RNase III domain of human Dicer

Journal Article · Sat Apr 01 04:00:00 EST 2006 · Acta Crystallographica. Section F

DOI:https://doi.org/10.1107/S174430910600889X· OSTI ID:22356348

Takeshita, Daijiro ^[1]; Zenno, Shuhei ^[2]; Lee, Woo Cheol; Nagata, Koji ^[1]; Saigo, Kaoru ^[2]; Tanokura, Masaru ^[1]

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1. Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 (Japan)
2. Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7-3-1 Hungo, Bunkyo-ku, Tokyo 113-0033 (Japan)

The C-terminal RNase III domain (RNase IIIb) of human Dicer has been expressed, purified and crystallized by the sitting-drop vapour-diffusion method. Human Dicer protein contains two RNase III domains (RNase IIIa and RNase IIIb) which are involved in the production of short interfering RNAs (siRNAs). The C-terminal RNase III domain (RNase IIIb) of human Dicer was expressed, purified and crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space group C222{sub 1}, with unit-cell parameters a = 88.6, b = 199.7, c = 119.6 Å, and diffracted X-rays to 2.0 Å resolution. The asymmetric unit contained three molecules of the RNase IIIb and the solvent content was 67%.

• OSTI ID: 22356348
• Journal Information: Acta Crystallographica. Section F, Journal Name: Acta Crystallographica. Section F Journal Issue: Pt 4 Vol. 62; ISSN ACSFCL; ISSN 1744-3091
• Country of Publication: United Kingdom
• Language: English

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