Preliminary X-ray investigations of several crystal forms of the ferripyoverdine FpvA outer membrane receptor from Pseudomonas aeruginosa bound to ferripyoverdine
- Institut Gilbert-Laustriat UMR 7175 CNRS/Université Strasbourg I, Département Récepteurs et Protéines Membranaires, Ecole Supérieure de Biotechnologie de Strasbourg, Boulevard Sébastien Brandt, BP 10413, F-67412 Illkirch (France)
The crystallization and X-ray data analysis of three crystal forms of the outer membrane pyoverdine transducer FpvA from P. aeruginosa bound to ferripyoverdine are described. The resolution of the crystals ranges from 3.15 to 2.7 Å depending on the crystal form; all were obtained in the presence of C{sub 8}E{sub 4} detergent. Ferripyoverdine transport across the outer membrane of Pseudomonas aeruginosa by the pyoverdine receptor FpvA and the transcriptional regulation of FpvA involve interactions of the FpvA N-terminal TonB box and signalling domain with proteins from the inner membrane. Several crystallization conditions of FpvA–Pvd-Fe solubilized in C{sub 8}E{sub 4} detergent were obtained and X-ray data were collected from three crystal forms. The resolution limits range from 3.15 to 2.7 Å depending on the crystal form. From preliminary analysis of the electron-density maps, the first full-length structure of an outer membrane receptor including a signalling domain should be determined.
- OSTI ID:
- 22356320
- Journal Information:
- Acta Crystallographica. Section F, Vol. 62, Issue Pt 5; Other Information: PMCID: PMC2219977; PMID: 16682776; PUBLISHER-ID: ll5058; OAI: oai:pubmedcentral.nih.gov:2219977; Copyright (c) International Union of Crystallography 2006; Country of input: International Atomic Energy Agency (IAEA); ISSN 1744-3091
- Country of Publication:
- United Kingdom
- Language:
- English
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