Crystallization and preliminary X-ray crystallographic studies of fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv
Journal Article
·
· Acta Crystallographica. Section F
- Biomedical Research Center, Division of Life Sicence, Korea Institute of Science and Technology, 39-1 Hawolgok-dong, Sungbuk-gu, Seoul 136-791 (Korea, Republic of)
- Division of Biotechnology and Genetic Engineering, Korea University, Seoul 136-701 (Korea, Republic of)
Fatty acid-CoA racemase from M. tuberculosis H37Rv has been overexpressed, purified and crystallized. Diffraction data have been collected to beyond 2.7 Å resolution using a synchrotron-radiation source. Fatty acid-CoA racemase plays an important role in the β-oxidation of branched-chain fatty acids and fatty-acid derivatives as it catalyzes the conversion of several (2R)-branched-chain fatty acid-CoAs to their (2S)-stereoisomers. Fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv has been purified to homogeneity and crystallized by the hanging-drop vapour-diffusion method with polyethylene glycol 4000 as precipitant. The crystals belong to the trigonal space group P3{sub 1} or P3{sub 2}, with unit-cell parameters a = b = 109.56, c = 147.97 Å. The asymmetric unit contains six monomers, corresponding to a V{sub M} value of 2.15 Å{sup 3} Da{sup −1}. A complete native data set has been collected at 2.7 Å resolution using a synchrotron-radiation source.
- OSTI ID:
- 22356172
- Journal Information:
- Acta Crystallographica. Section F, Journal Name: Acta Crystallographica. Section F Journal Issue: Pt 11 Vol. 61; ISSN ACSFCL; ISSN 1744-3091
- Country of Publication:
- United Kingdom
- Language:
- English
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