Crystallization and preliminary X-ray analysis of gene product 44 from bacteriophage Mu
- Institute for Protein Research, Osaka University, Yamada-oka, Suita 565-0871 (Japan)
- Department of Nano-Material Systems Graduate School of Engineering, Gunma University, 1-5-1 Tenjin-chyo, Kiryu, Gunma 376-8515 (Japan)
- Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Toyama (Japan)
Bacteriophage Mu baseplate protein gene product 44 was crystallized. The crystal belongs to space group R3, with unit-cell parameters a = b = 126.6, c = 64.2 Å. Bacteriophage Mu baseplate protein gene product 44 (gp44) is an essential protein required for the assembly of viable phages. To investigate the roles of gp44 in baseplate assembly and infection, gp44 was crystallized at pH 6.0 in the presence of 20% 2-methyl-2,4-pentanediol. The crystals belong to space group R3, with unit-cell parameters a = b = 127.47, c = 63.97 Å. The crystals diffract X-rays to at least 2.1 Å resolution and are stable in the X-ray beam and are therefore appropriate for structure determination. Native data have been collected to 2.1 Å resolution using a DIP6040 image-plate system at beamline BL44XU at the SPring-8 facility in Japan.
- OSTI ID:
- 22356090
- Journal Information:
- Acta Crystallographica. Section F, Vol. 61, Issue Pt 1; Other Information: PMCID: PMC1952399; PMID: 16508104; PUBLISHER-ID: en5076; OAI: oai:pubmedcentral.nih.gov:1952399; Copyright (c) International Union of Crystallography 2005; Country of input: International Atomic Energy Agency (IAEA); ISSN 1744-3091
- Country of Publication:
- United Kingdom
- Language:
- English
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