Crystallization and preliminary X-ray diffraction analysis of human growth and differentiation factor 5 (GDF-5)
Journal Article
·
· Acta Crystallographica. Section F
- Lehrstuhl für Physiologische Chemie II, Theodor-Boveri Institut für Biowissenschaften (Biozentrum) der Universität Würzburg, Am Hubland, D-97074 Würzburg (Germany)
Crystals of human growth and differentiation factor 5 are trigonal, belonging to space group P3{sub 1}21 or its enantiomer, with one molecule per asymmetric unit and diffract to 2.2 Å resolution. Growth and differentiation factor 5 (GDF-5) belongs to the large TGF-β superfamily of secreted signalling proteins and plays a pivotal role in skeletal development during embryogenesis. The gene for human GDF-5 was cloned, expressed in Escherichia coli and purified to homogeneity. Crystals were obtained that diffracted to 2.2 Å resolution. A native data set was acquired, showing that the crystals belong to a trigonal space group, i.e. P3{sub 1}21 or P3{sub 2}21, with unit-cell parameters a = b = 97.1, c = 48.3 Å. Initial analysis suggest the presence of only one monomer in the asymmetric unit, resulting in a high solvent content of 72% in the crystal.
- OSTI ID:
- 22356080
- Journal Information:
- Acta Crystallographica. Section F, Journal Name: Acta Crystallographica. Section F Journal Issue: Pt 1 Vol. 61; ISSN ACSFCL; ISSN 1744-3091
- Country of Publication:
- United Kingdom
- Language:
- English
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