Crystallization and preliminary X-ray crystallographic study of disproportionating enzyme from potato
Journal Article
·
· Acta Crystallographica. Section F
- Laboratory of Enzyme Chemistry, Graduate School of Agriculture and Biological Science, Osaka Prefecture University, Sakai, Osaka 599-8531 (Japan)
- Institute for Protein Research, Osaka University, Suita, Osaka 565-0871 (Japan)
- Biochemical Research Laboratory, Ezaki Glico Co. Ltd, Utajima, Nishiyodogawa-ku, Osaka 555-8502 (Japan)
Disproportionating enzyme from potato was crystallized and preliminarily analyzed using X-ray diffraction. Disproportionating enzyme (D-enzyme; EC 2.4.1.25) is a 59 kDa protein that belongs to the α-amylase family. D-enzyme catalyses intramolecular and intermolecular transglycosylation reactions of α-1,4 glucan. A crystal of the D-enzyme from potato was obtained by the hanging-drop vapour-diffusion method. Preliminary X-ray data showed that the crystal diffracts to 2.0 Å resolution and belongs to space group C222{sub 1}, with unit-cell parameters a = 69.7, b = 120.3, c = 174.2 Å.
- OSTI ID:
- 22356066
- Journal Information:
- Acta Crystallographica. Section F, Journal Name: Acta Crystallographica. Section F Journal Issue: Pt 1 Vol. 61; ISSN ACSFCL; ISSN 1744-3091
- Country of Publication:
- United Kingdom
- Language:
- English
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