Crystallization of a protein using dehydration without a precipitant

Journal Article · Wed Jun 01 00:00:00 EDT 2005 · Acta Crystallographica. Section F

DOI:https://doi.org/10.1107/S1744309105014235· OSTI ID:22356029

Baker, Edward N.; Lott, J. Shaun ^[1]

---

1. School of Biological Sciences and Centre for Molecular Biodiscovery, University of Auckland, Private Bag 92-019, Auckland 1003 (New Zealand)

Crystals grown by simple dehydration from a solution of hypoxic response protein I (HRPI) from M. tuberculosis diffract to a maximum resolution of 2.1 Å Hypoxic response protein I (HRPI) is a protein of unknown biochemical function whose expression is very strongly upregulated in response to oxygen depletion in Mycobacterium tuberculosis. Crystals have been grown from a solution of full-length HRPI by the unusual method of dehydration without the use of precipitants. The crystals produced diffract to a maximum resolution of 2.1 Å and belong to space group P4{sub 1}2{sub 1}2 (or P4{sub 3}2{sub 1}2), with unit-cell parameters a = b = 79.18, c = 37.34 Å.

• OSTI ID: 22356029
• Journal Information: Acta Crystallographica. Section F, Vol. 61, Issue Pt 6; Other Information: PMCID: PMC1952335; PMID: 16511097; PUBLISHER-ID: gx5055; OAI: oai:pubmedcentral.nih.gov:1952335; Copyright (c) International Union of Crystallography 2005; Country of input: International Atomic Energy Agency (IAEA); ISSN 1744-3091
• Country of Publication: United Kingdom
• Language: English

Related Subjects

75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
CRYSTALLIZATION
CRYSTALS
DEHYDRATION
LENGTH
MATHEMATICAL SOLUTIONS
OXYGEN
PROTEINS
RESOLUTION
SOLUTIONS
SPACE GROUPS