Crystallization and preliminary X-ray diffraction analysis of a chitin-binding domain of hyperthermophilic chitinase from Pyrococcus furiosus

Journal Article · Sun May 01 00:00:00 EDT 2005 · Acta Crystallographica. Section F

DOI:https://doi.org/10.1107/S1744309105010110· OSTI ID:22356008

Nakamura, Tsutomu; Ishikawa, Kazuhiko; Hagihara, Yoshihisa; Oku, Takashi ^[1]; Nakagawa, Atsushi ^[2]; Inoue, Tsuyoshi ^[3]; Ataka, Mitsuo; Uegaki, Koichi ^[1]

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1. National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577 (Japan)
2. Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871 (Japan)
3. Department of Materials Chemistry, Graduate School of Engineering, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871 (Japan)

The expression, purification and preliminary X-ray diffraction studies of a chitin-binding domain of the chitinase from P. furiosus are reported. The crystallization and preliminary X-ray diffraction analysis of the chitin-binding domain of chitinase from a hyperthermophilic archaeon, Pyrococcus furiosus, are reported. The recombinant protein was prepared using an Escherichia coli overexpression system and was crystallized by the hanging-drop vapour-diffusion method. An X-ray diffraction data set was collected to 1.70 Å resolution. The crystal belonged to space group P4{sub 3}2{sub 1}2 or P4{sub 1}2{sub 1}2. The unit-cell parameters were determined to be a = b = 48.8, c = 85.0 Å.

• OSTI ID: 22356008
• Journal Information: Acta Crystallographica. Section F, Vol. 61, Issue Pt 5; Other Information: PMCID: PMC1952313; PMID: 16511072; PUBLISHER-ID: za5096; OAI: oai:pubmedcentral.nih.gov:1952313; Copyright (c) International Union of Crystallography 2005; Country of input: International Atomic Energy Agency (IAEA); ISSN 1744-3091
• Country of Publication: United Kingdom
• Language: English

Related Subjects

75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
CRYSTALLIZATION
CRYSTALS
DIFFUSION
ESCHERICHIA COLI
PROTEINS
RESOLUTION
SPACE GROUPS
X-RAY DIFFRACTION