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Title: Expression, purification, crystallization and preliminary X-ray analysis of YaeQ (XAC2396) from Xanthomonas axonopodis pv. citri

Abstract

The first crystallographic study of a member of the YaeQ family of proteins, which are conserved in a small group of Gram-negative bacteria, most of which are animal or plant pathogens, is reported. Diffraction data were collected to 1.9 Å resolution and an interpretable electron-density map was obtained. Xanthomonas axonopodis pv. citri YaeQ (XAC2396) is a member of a family of bacterial proteins conserved in several Gram-negative pathogens. Here, the cloning, expression, purification and crystallization of the 182-residue (20.6 kDa) YaeQ protein are described. Recombinant YaeQ containing selenomethionine was crystallized in space group P2{sub 1} and crystals diffracted to 1.9 Å resolution at a synchrotron source. The unit-cell parameters are a = 39.75, b = 91.88, c = 48.03 Å, β = 108.37°. The calculated Matthews coefficient suggests the presence of two YaeQ molecules in the asymmetric unit. Initial experimental phases were calculated by the multiple-wavelength anomalous dispersion technique and an interpretable electron-density map was obtained.

Authors:
 [1];  [2];  [1]; ;
  1. Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, CEP 05508-000, São Paulo, SP (Brazil)
  2. Instituto de Física de São Carlos, Universidade de São Paulo, CEP 13560-970, São Carlos, SP (Brazil)
Publication Date:
OSTI Identifier:
22356006
Resource Type:
Journal Article
Journal Name:
Acta Crystallographica. Section F
Additional Journal Information:
Journal Volume: 61; Journal Issue: Pt 5; Other Information: PMCID: PMC1952311; PMID: 16511077; PUBLISHER-ID: en5110; OAI: oai:pubmedcentral.nih.gov:1952311; Copyright (c) International Union of Crystallography 2005; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 1744-3091
Country of Publication:
United Kingdom
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CRYSTALLIZATION; CRYSTALS; DENSITY; DIFFRACTION; DISPERSIONS; ELECTRON DENSITY; MOLECULES; PROTEINS; RESOLUTION; SPACE GROUPS; WAVELENGTHS

Citation Formats

Guzzo, Cristiane R., Nagem, Ronaldo A. P., Galvão-Botton, Leonor M. P., Guimarães, Beatriz G., Medrano, Francisco J., Barbosa, João A. R. G., E-mail: joao@lnls.br, and Farah, Chuck S., E-mail: joao@lnls.br. Expression, purification, crystallization and preliminary X-ray analysis of YaeQ (XAC2396) from Xanthomonas axonopodis pv. citri. United Kingdom: N. p., 2005. Web. doi:10.1107/S1744309105010985.
Guzzo, Cristiane R., Nagem, Ronaldo A. P., Galvão-Botton, Leonor M. P., Guimarães, Beatriz G., Medrano, Francisco J., Barbosa, João A. R. G., E-mail: joao@lnls.br, & Farah, Chuck S., E-mail: joao@lnls.br. Expression, purification, crystallization and preliminary X-ray analysis of YaeQ (XAC2396) from Xanthomonas axonopodis pv. citri. United Kingdom. https://doi.org/10.1107/S1744309105010985
Guzzo, Cristiane R., Nagem, Ronaldo A. P., Galvão-Botton, Leonor M. P., Guimarães, Beatriz G., Medrano, Francisco J., Barbosa, João A. R. G., E-mail: joao@lnls.br, and Farah, Chuck S., E-mail: joao@lnls.br. 2005. "Expression, purification, crystallization and preliminary X-ray analysis of YaeQ (XAC2396) from Xanthomonas axonopodis pv. citri". United Kingdom. https://doi.org/10.1107/S1744309105010985.
@article{osti_22356006,
title = {Expression, purification, crystallization and preliminary X-ray analysis of YaeQ (XAC2396) from Xanthomonas axonopodis pv. citri},
author = {Guzzo, Cristiane R. and Nagem, Ronaldo A. P. and Galvão-Botton, Leonor M. P. and Guimarães, Beatriz G. and Medrano, Francisco J. and Barbosa, João A. R. G., E-mail: joao@lnls.br and Farah, Chuck S., E-mail: joao@lnls.br},
abstractNote = {The first crystallographic study of a member of the YaeQ family of proteins, which are conserved in a small group of Gram-negative bacteria, most of which are animal or plant pathogens, is reported. Diffraction data were collected to 1.9 Å resolution and an interpretable electron-density map was obtained. Xanthomonas axonopodis pv. citri YaeQ (XAC2396) is a member of a family of bacterial proteins conserved in several Gram-negative pathogens. Here, the cloning, expression, purification and crystallization of the 182-residue (20.6 kDa) YaeQ protein are described. Recombinant YaeQ containing selenomethionine was crystallized in space group P2{sub 1} and crystals diffracted to 1.9 Å resolution at a synchrotron source. The unit-cell parameters are a = 39.75, b = 91.88, c = 48.03 Å, β = 108.37°. The calculated Matthews coefficient suggests the presence of two YaeQ molecules in the asymmetric unit. Initial experimental phases were calculated by the multiple-wavelength anomalous dispersion technique and an interpretable electron-density map was obtained.},
doi = {10.1107/S1744309105010985},
url = {https://www.osti.gov/biblio/22356006}, journal = {Acta Crystallographica. Section F},
issn = {1744-3091},
number = Pt 5,
volume = 61,
place = {United Kingdom},
year = {Sun May 01 00:00:00 EDT 2005},
month = {Sun May 01 00:00:00 EDT 2005}
}