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Title: Cloning, expression, purification, crystallization and initial crystallographic analysis of transcription elongation factors GreB from Escherichia coli and Gfh1 from Thermus thermophilus

Abstract

The GreB transcription cleavage factor of E. coli and the Gfh1 transcription elongation factor of T. thermophilus were cloned, expressed, purified and crystallized. Complete diffraction data sets were collected for the GreB and Gfh1 crystals to 2.6 and 2.8 Å resolution, respectively. Structure determination of these proteins is now in progress.

Authors:
; ; ; ; ;
Publication Date:
OSTI Identifier:
22355954
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallographica. Section F; Journal Volume: 62; Journal Issue: Pt 1; Other Information: PMCID: PMC1401493; PMID: 16511259; PUBLISHER-ID: pu5114; OAI: oai:pubmedcentral.nih.gov:1401493; Copyright (c) International Union of Crystallography 2006; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United Kingdom
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CLEAVAGE; CRYSTALLIZATION; CRYSTALS; DIFFRACTION; ELONGATION; ESCHERICHIA COLI; PROTEINS; RESOLUTION

Citation Formats

Perederina, Anna A., Vassylyeva, Marina N., Berezin, Igor A., Svetlov, Vladimir, Artsimovitch, Irina, and Vassylyev, Dmitry G., E-mail: dmitry@uab.edu. Cloning, expression, purification, crystallization and initial crystallographic analysis of transcription elongation factors GreB from Escherichia coli and Gfh1 from Thermus thermophilus. United Kingdom: N. p., 2006. Web. doi:10.1107/S1744309105040297.
Perederina, Anna A., Vassylyeva, Marina N., Berezin, Igor A., Svetlov, Vladimir, Artsimovitch, Irina, & Vassylyev, Dmitry G., E-mail: dmitry@uab.edu. Cloning, expression, purification, crystallization and initial crystallographic analysis of transcription elongation factors GreB from Escherichia coli and Gfh1 from Thermus thermophilus. United Kingdom. doi:10.1107/S1744309105040297.
Perederina, Anna A., Vassylyeva, Marina N., Berezin, Igor A., Svetlov, Vladimir, Artsimovitch, Irina, and Vassylyev, Dmitry G., E-mail: dmitry@uab.edu. Sun . "Cloning, expression, purification, crystallization and initial crystallographic analysis of transcription elongation factors GreB from Escherichia coli and Gfh1 from Thermus thermophilus". United Kingdom. doi:10.1107/S1744309105040297.
@article{osti_22355954,
title = {Cloning, expression, purification, crystallization and initial crystallographic analysis of transcription elongation factors GreB from Escherichia coli and Gfh1 from Thermus thermophilus},
author = {Perederina, Anna A. and Vassylyeva, Marina N. and Berezin, Igor A. and Svetlov, Vladimir and Artsimovitch, Irina and Vassylyev, Dmitry G., E-mail: dmitry@uab.edu},
abstractNote = {The GreB transcription cleavage factor of E. coli and the Gfh1 transcription elongation factor of T. thermophilus were cloned, expressed, purified and crystallized. Complete diffraction data sets were collected for the GreB and Gfh1 crystals to 2.6 and 2.8 Å resolution, respectively. Structure determination of these proteins is now in progress.},
doi = {10.1107/S1744309105040297},
journal = {Acta Crystallographica. Section F},
number = Pt 1,
volume = 62,
place = {United Kingdom},
year = {Sun Jan 01 00:00:00 EST 2006},
month = {Sun Jan 01 00:00:00 EST 2006}
}