Experimental phasing using zinc anomalous scattering

Journal Article · Sat Sep 01 00:00:00 EDT 2012 · Acta Crystallographica. Section D: Biological Crystallography

DOI:https://doi.org/10.1107/S0907444912024420· OSTI ID:22347865

Cha, Sun-Shin ^[1]; An, Young Jun; Jeong, Chang-Sook; Kim, Min-Kyu ^[1]; Lee, Sung-Gyu; Lee, Kwang-Hoon; Oh, Byung-Ha ^[2]

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1. Korea Institute of Ocean Science and Technology, Ansan 426-744 (Korea, Republic of)
2. Korea Advanced Institute of Science and Technology, Daejeon 305-701 (Korea, Republic of)

The surface of proteins can be charged with zinc ions and the anomalous signals from these zinc ions can be used for structure determination of proteins. Zinc is a suitable metal for anomalous dispersion phasing methods in protein crystallography. Structure determination using zinc anomalous scattering has been almost exclusively limited to proteins with intrinsically bound zinc(s). Here, it is reported that multiple zinc ions can easily be charged onto the surface of proteins with no intrinsic zinc-binding site by using zinc-containing solutions. Zn derivatization of protein surfaces appears to be a largely unnoticed but promising method of protein structure determination.

• OSTI ID: 22347865
• Journal Information: Acta Crystallographica. Section D: Biological Crystallography, Vol. 68, Issue Pt 9; Other Information: PMCID: PMC3489106; PMID: 22948927; PUBLISHER-ID: wd5183; OAI: oai:pubmedcentral.nih.gov:3489106; Copyright (c) Cha et al. 2012; This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0907-4449
• Country of Publication: Denmark
• Language: English

Related Subjects

75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
CRYSTALLOGRAPHY
CRYSTALS
DERIVATIZATION
MATHEMATICAL SOLUTIONS
PROTEIN STRUCTURE
PROTEINS
SCATTERING
SIGNALS
SOLUTIONS
SURFACES
ZINC
ZINC IONS