Supplemental defatted microalgae affects egg and tissue fatty acid composition differently in laying hens fed diets containing corn and flaxseed oil

Kim, Jonggun; Barcus, Matthew; Magnuson, Andrew; Tao, Ling; Lei, Xin Gen

doi:10.3382/japr/pfw034

Title: Supplemental defatted microalgae affects egg and tissue fatty acid composition differently in laying hens fed diets containing corn and flaxseed oil

Journal Article · Wed Dec 11 00:00:00 EST 2019 · Journal of Applied Poultry Research

DOI:https://doi.org/10.3382/japr/pfw034· OSTI ID:2229725

Kim, Jonggun ^[1]; Barcus, Matthew ^[1]; Magnuson, Andrew ^[1]; Tao, Ling ^[1]; Lei, Xin Gen ^[1]

Cornell University, Ithaca, NY (United States)

Effects of defatted microalgae (DFA) on fatty acid composition of eggs and tissues of hens have not been well studied. This experiment was to determine how low concentrations of supplemental DFA in the presence or absence of flaxseed oil (FO) altered fatty acid profiles of egg yolk, liver, adipose tissue, and plasma of layers. A total of 60 Shaver Leghorn layer hens (individually caged, 20-wk old) were allotted into 6 groups (n = 10) and fed a corn/soybean meal basal diet containing 0, 3, or 5% DFA and 0 (1.5% corn oil, CO) or 1.5% FO for 6 wk. BW, ADFI, egg production rate, and egg component weights were not affected by any of the dietary treatments at various time points, but egg yolk color was changed (P < 0.05) with increasing concentrations of DFA. The concentration of n-3 fatty acids in egg yolk and plasma were elevated by FO up to 9-fold (P < 0.05) starting at wk 1 or 2, and 39 to 83% (P < 0.05) by DFA. Supplemental DFA improved (P < 0.05) n-6/n-3 fatty acid ratios in egg yolk and plasma from 13 to 23 and 7 to 13, respectively, only in hens fed diets without FO. Fatty acid profiles of liver and adipose tissue (at wk 6) displayed responses to supplemental DFA and FO similar to those of egg yolk or plasma. In conclusion, supplemental 3 or 5% DFA caused moderate enrichments of n-3 fatty acids and decreased the n6/n3 fatty acid ratios of egg yolk and tissues in hens fed diets without FO.

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Research Organization:: Cornell Univ., Ithaca, NY (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Office of Technology Development (EE-20)

Grant/Contract Number:: EE0007091

OSTI ID:: 2229725

Journal Information:: Journal of Applied Poultry Research, Vol. 25, Issue 4; ISSN 1056-6171

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

References (44)

Significance of the N‐6/N‐3 Ratio for Insulin Action in Diabetes Raheja, Bihari S.; Sadikot, Shaukat M.; Phatak, Raghunath B. Annals of the New York Academy of Sciences, Vol. 683, Issue 1 https://doi.org/10.1111/j.1749-6632.1993.tb35715.x	journal	June 1993
Patterns of Lipid Storage and Utilization in Birds Blem, Charles R. American Zoologist, Vol. 16, Issue 4 https://doi.org/10.1093/icb/16.4.671	journal	November 1976
Nutritional and Metabolic Impacts of a Defatted Green Marine Microalgal ( Desmodesmus sp.) Biomass in Diets for Weanling Pigs and Broiler Chickens Ekmay, Ricardo; Gatrell, Stephanie; Lum, Krystal Journal of Agricultural and Food Chemistry, Vol. 62, Issue 40 https://doi.org/10.1021/jf501155n	journal	September 2014
Creating ω-3 Fatty-Acid-Enriched Chicken Using Defatted Green Microalgal Biomass Gatrell, Stephanie K.; Kim, JongGun; Derksen, Theodore J. Journal of Agricultural and Food Chemistry, Vol. 63, Issue 42 https://doi.org/10.1021/acs.jafc.5b03137	journal	October 2015
The fate of plasma free fatty acids and their effects on tissue metabolism Steinberg, Daniel Metabolism, Vol. 13, Issue 10 https://doi.org/10.1016/S0026-0495(64)80043-3	journal	October 1964
Omega-3 Fatty Acid Biochemistry: Perspectives from Human Nutrition Innis, Sheila M. Military Medicine, Vol. 179, Issue 11S https://doi.org/10.7205/MILMED-D-14-00147	journal	November 2014
Manipulation of then-3 polyunsaturated fatty acid composition of avian eggs and meat Leskanich, C. O.; Noble, R. C. World's Poultry Science Journal, Vol. 53, Issue 2 https://doi.org/10.1079/WPS19970015	journal	June 1997
Micro-algae as a source of protein Becker, E. W. Biotechnology Advances, Vol. 25, Issue 2 https://doi.org/10.1016/j.biotechadv.2006.11.002	journal	March 2007
Liver Synthesis, Plasma Transport, and Structural Alterations Accompanying Passage of yolk Proteins Schjeide, O. A.; Wilkens, Myrna; McCANDLESS, Ruth G. American Zoologist, Vol. 3, Issue 2 https://doi.org/10.1093/icb/3.2.167	journal	May 1963
Feeding Laying Hens Stearidonic Acid-Enriched Soybean Oil, as Compared to Flaxseed Oil, More Efficiently Enriches Eggs with Very Long-Chain n-3 Polyunsaturated Fatty Acids Elkin, Robert G.; Ying, Yun; Harvatine, Kevin J. Journal of Agricultural and Food Chemistry, Vol. 63, Issue 10 https://doi.org/10.1021/jf505185u	journal	March 2015
N‐3 and N‐6 fatty acids in breast adipose tissue and relative risk of breast cancer in a case‐control study in Tours, France Maillard, Virginie; Bougnoux, Philippe; Ferrari, Pietro International Journal of Cancer, Vol. 98, Issue 1 https://doi.org/10.1002/ijc.10130	journal	December 2001
Enriched Eggs as a Source of N-3 Polyunsaturated Fatty Acids for Humans Lewis, N. M.; Seburg, S.; Flanagan, N. L. Poultry Science, Vol. 79, Issue 7 https://doi.org/10.1093/ps/79.7.971	journal	July 2000
Understanding omega-3's DeFilippis, Andrew P.; Sperling, Laurence S. American Heart Journal, Vol. 151, Issue 3 https://doi.org/10.1016/j.ahj.2005.03.051	journal	March 2006
Effect of dietary defatted diatom biomass on egg production and quality of laying hens Leng, Xiangjun; Hsu, Kun-Nan; Austic, Richard E. Journal of Animal Science and Biotechnology, Vol. 5, Issue 1 https://doi.org/10.1186/2049-1891-5-3	journal	January 2014
Effects of dietary algal docosahexaenoic acid oil supplementation on fatty acid deposition and gene expression in laying Leghorn hens Cheng, C. H.; Shen, T. F.; Chen, W. L. The Journal of Agricultural Science, Vol. 142, Issue 6 https://doi.org/10.1017/S0021859605004867	journal	December 2004
Response of Layers to Dietary Flaxseed According to Body Weight Classification at Maturity Leeson, S.; Summers, J. D.; Caston, L. J. Journal of Applied Poultry Research, Vol. 9, Issue 3 https://doi.org/10.1093/japr/9.3.297	journal	October 2000
Commercial applications of microalgae Spolaore, Pauline; Joannis-Cassan, Claire; Duran, Elie Journal of Bioscience and Bioengineering, Vol. 101, Issue 2 https://doi.org/10.1263/jbb.101.87	journal	February 2006
Distribution, interconversion, and dose response of n−3 fatty acids in humans Arterburn, Linda M.; Hall, Eileen Bailey; Oken, Harry The American Journal of Clinical Nutrition, Vol. 83, Issue 6 https://doi.org/10.1093/ajcn/83.6.1467S	journal	June 2006
Potential and Limitation of a New Defatted Diatom Microalgal Biomass in Replacing Soybean Meal and Corn in Diets for Broiler Chickens Austic, Richard E.; Mustafa, Ahsan; Jung, Byoungyun Journal of Agricultural and Food Chemistry, Vol. 61, Issue 30 https://doi.org/10.1021/jf401957z	journal	July 2013
Trans Fatty Acids in Adipose Tissue and the Food Supply Are Associated with Myocardial Infarction Clifton, Peter M.; Keogh, Jennifer B.; Noakes, Manny The Journal of Nutrition, Vol. 134, Issue 4 https://doi.org/10.1093/jn/134.4.874	journal	April 2004
Conversion of α -linolenic acid to longer-chain polyunsaturated fatty acids in human adults Burdge, Graham C.; Calder, Philip C. Reproduction Nutrition Development, Vol. 45, Issue 5 https://doi.org/10.1051/rnd:2005047	journal	September 2005
Difference in Metabolism of Very Low Density Lipoprotein from Laying Chicken Hens in Comparison to Immature Chicken Hens Bacon, Wayne L.; Leclercq, Bernard; Blum, Jean-Claude Poultry Science, Vol. 57, Issue 6 https://doi.org/10.3382/ps.0571675	journal	November 1978
Dietary marine algae maintains egg consumer acceptability while enhancing yolk color Herber-McNeill, S. M.; Van Elswyk, M. E. Poultry Science, Vol. 77, Issue 3 https://doi.org/10.1093/ps/77.3.493	journal	March 1998
The Combined Influence of Dietary Flaxseed Variety, Level, Form, and Storage Conditions on Egg Production and Composition Among Vitamin E-Supplemented Hens Scheideler, S. E.; Froning, G. W. Poultry Science, Vol. 75, Issue 10 https://doi.org/10.3382/ps.0751221	journal	October 1996
Potential of combining flaxseed oil and microalgal biomass in producing eggs-enriched with n − 3 fatty acids for meeting human needs Kim, Jonggun; Magnuson, Andrew; Tao, Ling Algal Research, Vol. 17 https://doi.org/10.1016/j.algal.2016.04.005	journal	July 2016
Spirulina (Arthrospira): potential application as an animal feed supplement Belay, Amha; Kato, Toshimitsu; Ota, Yoshimichi Journal of Applied Phycology, Vol. 8, Issue 4-5 https://doi.org/10.1007/BF02178573	journal	July 1996
Continual feeding of two types of microalgal biomass affected protein digestion and metabolism in laying hens1 Ekmay, R. D.; Chou, K.; Magnuson, A. Journal of Animal Science, Vol. 93, Issue 1 https://doi.org/10.2527/jas.2014-7506	journal	January 2015
The nutritional value of algae for poultry. Dried chlorella in layer diets Lipstein, Bianka; Hurwitz, S.; Bornstein, S. British Poultry Science, Vol. 21, Issue 1 https://doi.org/10.1080/00071668008416631	journal	January 1980
Health Claims: Omega-3 Fatty Acids and Cardiovascular Disease Leaf, Alexander Nutrition Reviews, Vol. 50, Issue 5 https://doi.org/10.1111/j.1753-4887.1992.tb01310.x	journal	April 2009
Polyunsaturated fatty acids in the food chain in the United States Kris-Etherton, Pm; Taylor, Denise Shaffer; Yu-Poth, Shaomei The American Journal of Clinical Nutrition, Vol. 71, Issue 1 https://doi.org/10.1093/ajcn/71.1.179S	journal	January 2000
A Simple Method for the Isolation and Purification of Total Lipides from Animal Tissues Folch, Jordi; Lees, M.; Stanley, G. H. Sloane Journal of Biological Chemistry, Vol. 226, Issue 1 https://doi.org/10.1016/S0021-9258(18)64849-5	journal	May 1957
Functional Characterization of the Chicken Fatty Acid Elongases Gregory, Melissa K.; Geier, Mark S.; Gibson, Robert A. The Journal of Nutrition, Vol. 143, Issue 1 https://doi.org/10.3945/jn.112.170290	journal	November 2012
The Nutritional Value of Dehydrated, Blue-Green Algae (Spirulina plantensis) for Poultry Ross, Ernest; Dominy, Warren Poultry Science, Vol. 69, Issue 5 https://doi.org/10.3382/ps.0690794	journal	May 1990
Introduction Catapano, Alberico L. Atherosclerosis Supplements, Vol. 14, Issue 2 https://doi.org/10.1016/S1567-5688(13)70002-3	journal	August 2013
Effect of dietary fish oil on .omega.-3 fatty acid levels in chicken eggs and thigh flesh Huang, Zhibin; Leibovitz, Henry; Lee, Chong M. Journal of Agricultural and Food Chemistry, Vol. 38, Issue 3 https://doi.org/10.1021/jf00093a034	journal	March 1990
An Outlook on Microalgal Biofuels Wijffels, R. H.; Barbosa, M. J. Science, Vol. 329, Issue 5993, p. 796-799 https://doi.org/10.1126/science.1189003	journal	August 2010
Fatty acid profile of egg yolk lipids from hens fed diets rich in n-3 fatty acids Milinsk, M. C.; Murakami, A. E.; Gomes, S. T. M. Food Chemistry, Vol. 83, Issue 2 https://doi.org/10.1016/S0308-8146(03)00094-3	journal	November 2003
Effect of dietary flaxseed, flax oil and n-3 fatty acid supplement on hepatic and plasma characteristics relevant to fatty liver haemorrhagic syndrome in laying hens Schumann, B. E.; Squires, E. J.; Leeson, S. British Poultry Science, Vol. 41, Issue 4 https://doi.org/10.1080/713654970	journal	September 2000
The importance of the ratio of omega-6/omega-3 essential fatty acids Simopoulos, A. P. Biomedicine & Pharmacotherapy, Vol. 56, Issue 8 https://doi.org/10.1016/S0753-3322(02)00253-6	journal	October 2002
Atherosclerosis — An Inflammatory Disease Ross, Russell New England Journal of Medicine, Vol. 340, Issue 2 https://doi.org/10.1056/NEJM199901143400207	journal	January 1999
Hepatic lipid characteristics and histopathology of laying hens fed CLA or n−3 fatty acids Cherian, Gita; Goeger, Mary P. Lipids, Vol. 39, Issue 1 https://doi.org/10.1007/s11745-004-1198-2	journal	January 2004
Fatty acid and carotenoid composition of egg yolk as an effect of microalgae addition to feed formula for laying hens Fredriksson, Sofia; Elwinger, Klas; Pickova, Jana Food Chemistry, Vol. 99, Issue 3 https://doi.org/10.1016/j.foodchem.2005.08.018	journal	January 2006
In Vivo Lipogenesis in the Domestic Chicken Leveille, G. A.; O'Hea, E. K.; Chakrabarty, K. Experimental Biology and Medicine, Vol. 128, Issue 2 https://doi.org/10.3181/00379727-128-33022	journal	June 1968
Evaluation of a Food Frequency Questionnaire with Weighed Records, Fatty Acids, and Alpha-Tocopherol in Adipose Tissue and Serum Andersen, L. F.; Solvoll, K.; Johansson, L. R. K. American Journal of Epidemiology, Vol. 150, Issue 1 https://doi.org/10.1093/oxfordjournals.aje.a009921	journal	July 1999

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