Inorganic voltage nanosensors for bioelectricity studies in bacterial communities
- University of California, Los Angeles, CA (United States); UCLA
We developed both membrane potential nanosensors (MPNs) and voltage sensing dyes (VSDs), and quantitative optical measurement tools to measure membrane potential (MP) at the level of a single bacterial cell, with voltage resolution of ~10 mV changes. Our approach relied on (i) novel MPNs and novel VSDs that measures MPs via fluorescence lifetime; (ii) a quantitative approach to fluorescence lifetime imaging microscopy (FLIM) data based on a novel time-resolved camera and phasor analysis. We demonstrate MP measurements over time for single bacterial cells.
- Research Organization:
- University of California, Los Angeles, CA (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- DOE Contract Number:
- SC0020338
- OSTI ID:
- 2229451
- Report Number(s):
- DOE-UCLA--11111-1
- Country of Publication:
- United States
- Language:
- English
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