High Affinity Electrostatic Interactions Support the Formation of CdS Quantum Dot:Nitrogenase MoFe Protein Complexes

Pellows, Lauren M.; Willis, Mark A.; Ruzicka, Jesse L.; Jagilinki, Bhanu P.; Mulder, David W.; Yang, Zhi-Yong; Seefeldt, Lance C.; King, Paul W.; Dukovic, Gordana; Peters, John W.

doi:10.1021/acs.nanolett.3c03205

High Affinity Electrostatic Interactions Support the Formation of CdS Quantum Dot:Nitrogenase MoFe Protein Complexes

Journal Article · Mon Nov 06 00:00:00 EST 2023 · Nano Letters

DOI:https://doi.org/10.1021/acs.nanolett.3c03205· OSTI ID:2222419

^[1]; Willis, Mark A. ^[2]; Ruzicka, Jesse L. ^[1]; ^[3]; ^[4]; ^[5]; ^[5]; ^[4]; ^[1]; Peters, John W. ^[6]

University of Colorado, Boulder, CO (United States)
Washington State University, Pullman, WA (United States)
University of Oklahoma, Norman, OK (United States)
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Utah State University, Logan, UT (United States)
Washington State University, Pullman, WA (United States); University of Oklahoma, Norman, OK (United States)

Nitrogenase MoFe protein can be coupled with CdS nanocrystals (NCs) to enable photocatalytic N₂ reduction. The nature of interactions that support complex formation is of paramount importance in intermolecular electron transfer that supports catalysis. In this work we have employed microscale thermophoresis to examine binding interactions between 3-mercaptopropionate capped CdS quantum dots (QDs) and MoFe protein over a range of QD diameters (3.4-4.3 nm). The results indicate that the interactions are largely electrostatic, with the strength of interactions similar to that observed for the physiological electron donor. In addition, the strength of interactions is sensitive to the QD diameter, and the binding interactions are significantly stronger for QDs with smaller diameters. Furthermore, the ability to quantitatively assess NC protein interactions in biohybrid systems supports strategies for understanding properties and reaction parameters that are important for obtaining optimal rates of catalysis in biohybrid systems.

View Accepted Manuscript (DOE)

Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 2222419

Report Number(s):: NREL/JA-2700-87515; MainId:88290; UUID:03eda84d-8b85-450a-998b-0e539c9ffa88; MainAdminID:71143

Journal Information:: Nano Letters, Journal Name: Nano Letters Journal Issue: 22 Vol. 23; ISSN 1530-6984

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: English

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High Affinity Electrostatic Interactions Support the Formation of CdS Quantum Dot:Nitrogenase MoFe Protein Complexes

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