Scar-less multi-part DNA assembly design automation

Hillson, Nathan J.

Title: Scar-less multi-part DNA assembly design automation

Patent · Tue Sep 05 00:00:00 EDT 2023

OSTI ID:2222290

Hillson, Nathan J.

The present invention provides a method of a method of designing an implementation of a DNA assembly. In an exemplary embodiment, the method includes (1) receiving a list of DNA sequence fragments to be assembled together and an order in which to assemble the DNA sequence fragments, (2) designing DNA oligonucleotides (oligos) for each of the DNA sequence fragments, and (3) creating a plan for adding flanking homology sequences to each of the DNA oligos. In an exemplary embodiment, the method includes (1) receiving a list of DNA sequence fragments to be assembled together and an order in which to assemble the DNA sequence fragments, (2) designing DNA oligonucleotides (oligos) for each of the DNA sequence fragments, and (3) creating a plan for adding optimized overhang sequences to each of the DNA oligos.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC02-05CH11231

Assignee:: The Regents of the University of California (Oakland, CA)

Patent Number(s):: 11,749,379

Application Number:: 16/435,406

OSTI ID:: 2222290

Resource Relation:: Patent File Date: 06/07/2019

Country of Publication:: United States

Language:: English

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Scar-less multi-part DNA assembly design automation Hillson, Nathan J. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/10373703 US Patent Document 10,373,703	patent	August 2019
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Scar-less multi-part DNA assembly design automation Hillson, Nathan J. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/9361427 US Patent Document 9,361,427	patent	June 2016
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Scar-Less Multi-Part DNA Assembly Design Automation Hillson, Nathan J. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20120259607 US Patent Application 13/364285; 20120259607	patent-application	October 2012

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