Massively parallel, multiple-organ perfusion control system

Reiserer, Ronald S.; Gerken, Gregory B.; Schaffer, David K.; Wikswo, John P.

Massively parallel, multiple-organ perfusion control system

Patent · Tue Sep 05 00:00:00 EDT 2023

OSTI ID:2222273

Reiserer, Ronald S.; Gerken, Gregory B.; Schaffer, David K.; Wikswo, John P.

A fluidic cartridge comprises a fluidic disk having a plurality of alignment openings; a fluidic chip comprising a body, one or more channels formed in the body in fluidic communications with input ports and output ports for transferring one or more fluids between the input ports and the output ports, and a plurality of protrusions formed on the body and received in the alignment openings of the fluidic disk for aligning the fluidic chip to the fluidic disk; an actuator operably engaging with the one or more channels for selectively and individually transferring the one or more fluids through the one or more channels from at least one of the input ports to at least one of the output ports at desired flow rates; and a tube member defining a cylindrical housing for accommodating the fluidic disk, the fluidic chip and the actuator therein.

View Patent

Research Organization:: Vanderbilt Univ., Nashville, TN (United States)

Sponsoring Organization:: USDOE; National Institutes of Health (NIH); National Aeronautics and Space Administration (NASA)

Assignee:: Vanderbilt University (Nashville, TN)

Patent Number(s):: 11,746,317

Application Number:: 17/984,151

OSTI ID:: 2222273

Country of Publication:: United States

Language:: English

References (62)

Real time monitoring of multiple parameters in mammalian cell culture bioreactors using an in-line Raman spectroscopy probe Abu-Absi, Nicholas R.; Kenty, Brian M.; Cuellar, Maryann Ehly Biotechnology and Bioengineering, Vol. 108, Issue 5 https://doi.org/10.1002/bit.23023	journal	December 2010
The future of industrial bioprocessing: Batch or continuous?: The Future of Industrial Bioprocessing Croughan, Matthew S.; Konstantinov, Konstantin B.; Cooney, Charles Biotechnology and Bioengineering, Vol. 112, Issue 4 https://doi.org/10.1002/bit.25529	journal	February 2015
Raman spectroscopy as a method to replace off‐line pH during mammalian cell culture processes Rafferty, Carl; O'Mahony, Jim; Burgoyne, Barbara Biotechnology and Bioengineering, Vol. 117, Issue 1 https://doi.org/10.1002/bit.27197	journal	November 2019
In situ Raman spectroscopy for simultaneous monitoring of multiple process parameters in mammalian cell culture bioreactors Whelan, Jessica; Craven, Stephen; Glennon, Brian Biotechnology Progress, Vol. 28, Issue 5 https://doi.org/10.1002/btpr.1590	journal	July 2012
Alternating flow filtration as an alternative to internal spin filter based perfusion process: Impact on productivity and product quality Bosco, Belén; Paillet, Cristian; Amadeo, Ignacio Biotechnology Progress, Vol. 33, Issue 4 https://doi.org/10.1002/btpr.2487	journal	May 2017
Analysis of chemometric models applied to Raman spectroscopy for monitoring key metabolites of cell culture Rafferty, Carl; Johnson, Kjell; O'Mahony, Jim Biotechnology Progress, Vol. 36, Issue 4 https://doi.org/10.1002/btpr.2977	journal	February 2020
Ultrasonic particle-concentration for sheathless focusing of particles for analysis in a flow cytometer Goddard, Gregory; Martin, John C.; Graves, Steven W. Cytometry Part A, Vol. 69A, Issue 2, p. 66-74 https://doi.org/10.1002/cyto.a.20205	journal	January 2006
Electrochemical Monitoring of Cellular Metabolism McKenzie, Jennifer R.; Cliffel, David E.; Wikswo, John P. Encyclopedia of Applied Electrochemistry, p. 522-528 https://doi.org/10.1007/978-1-4419-6996-5_263	book	January 2014
Quantitative monitoring of yeast fermentation using Raman spectroscopy Iversen, Jens A.; Berg, Rolf W.; Ahring, Birgitte K. Analytical and Bioanalytical Chemistry, Vol. 406, Issue 20 https://doi.org/10.1007/s00216-014-7897-2	journal	July 2014
Variation in diffusion of gases through PDMS due to plasma surface treatment and storage conditions Markov, Dmitry A.; Lillie, Elizabeth M.; Garbett, Shawn P. Biomedical Microdevices, Vol. 16, Issue 1 https://doi.org/10.1007/s10544-013-9808-2	journal	September 2013
Modeling the measurements of cellular fluxes in microbioreactor devices using thin enzyme electrodes Velkovsky, Momchil; Snider, Rachel; Cliffel, David E. Journal of Mathematical Chemistry, Vol. 49, Issue 1 https://doi.org/10.1007/s10910-010-9744-9	journal	October 2010
Modification of the Cytosensor™ microphysiometer to simultaneously measure extracellular acidification and oxygen consumption rates Eklund, Sven E.; Cliffel, David E.; Kozlov, Eugene Analytica Chimica Acta, Vol. 496, Issue 1-2 https://doi.org/10.1016/S0003-2670(03)00992-9	journal	October 2003
Characterization and comparison of ATF and TFF in stirred bioreactors for continuous mammalian cell culture processes Karst, Daniel J.; Serra, Elisa; Villiger, Thomas K. Biochemical Engineering Journal, Vol. 110 https://doi.org/10.1016/j.bej.2016.02.003	journal	June 2016
Perfusion mammalian cell culture for recombinant protein manufacturing – A critical review Bielser, Jean-Marc; Wolf, Moritz; Souquet, Jonathan Biotechnology Advances, Vol. 36, Issue 4 https://doi.org/10.1016/j.biotechadv.2018.04.011	journal	July 2018
SPE-IMS-MS: An automated platform for sub-sixty second surveillance of endogenous metabolites and xenobiotics in biofluids Zhang, Xing; Romm, Michelle; Zheng, Xueyun Clinical Mass Spectrometry, Vol. 2 https://doi.org/10.1016/j.clinms.2016.11.002	journal	December 2016
Continuous integrated manufacturing of therapeutic proteins Karst, Daniel Johannes; Steinebach, Fabian; Morbidelli, Massimo Current Opinion in Biotechnology, Vol. 53 https://doi.org/10.1016/j.copbio.2017.12.015	journal	October 2018
Shear contributions to cell culture performance and product recovery in ATF and TFF perfusion systems Wang, Samantha; Godfrey, Scott; Ravikrishnan, Janani Journal of Biotechnology, Vol. 246 https://doi.org/10.1016/j.jbiotec.2017.01.020	journal	March 2017
Multianalyte microphysiometry as a tool in metabolomics and systems biology Eklund, Sven E.; Snider, Rachel M.; Wikswo, John Journal of Electroanalytical Chemistry, Vol. 587, Issue 2 https://doi.org/10.1016/j.jelechem.2005.11.024	journal	February 2006
Microfluidic systems for extracting nucleic acids for DNA and RNA analysis Hui, Wing C.; Yobas, Levent; Samper, Victor D. Sensors and Actuators A: Physical, Vol. 133, Issue 2 https://doi.org/10.1016/j.sna.2006.06.031	journal	February 2007
Multichamber multipotentiostat system for cellular microphysiometry Lima, Eduardo A.; Snider, Rachel M.; Reiserer, Ronald S. Sensors and Actuators B: Chemical, Vol. 204 https://doi.org/10.1016/j.snb.2014.07.126	journal	December 2014
A bistable, multiport valve enables microformulators creating microclinical analyzers that reveal aberrant glutamate metabolism in astrocytes derived from a tuberous sclerosis patient Miller, Dusty R.; Schaffer, David K.; Neely, M. Diana Sensors and Actuators B: Chemical, Vol. 341 https://doi.org/10.1016/j.snb.2021.129972	journal	August 2021
A Dual-Column Solid Phase Extraction Strategy for Online Collection and Preparation of Continuously Flowing Effluent Streams for Mass Spectrometry Enders, Jeffrey R.; Marasco, Christina C.; Wikswo, John P. Analytical Chemistry, Vol. 84, Issue 20 https://doi.org/10.1021/ac3021032	journal	September 2012
Conformational Ordering of Biomolecules in the Gas Phase: Nitrogen Collision Cross Sections Measured on a Prototype High Resolution Drift Tube Ion Mobility-Mass Spectrometer May, Jody C.; Goodwin, Cody R.; Lareau, Nichole M. Analytical Chemistry, Vol. 86, Issue 4, p. 2107-2116 https://doi.org/10.1021/ac4038448	journal	February 2014
Real-Time Monitoring of Cellular Bioenergetics with a Multianalyte Screen-Printed Electrode McKenzie, Jennifer R.; Cognata, Andrew C.; Davis, Anna N. Analytical Chemistry, Vol. 87, Issue 15 https://doi.org/10.1021/acs.analchem.5b01533	journal	July 2015
An Integrated, High-Throughput Strategy for Multiomic Systems Level Analysis Gutierrez, Danielle B.; Gant-Branum, Randi L.; Romer, Carrie E. Journal of Proteome Research, Vol. 17, Issue 10 https://doi.org/10.1021/acs.jproteome.8b00302	journal	August 2018
Some myths and messages concerning the batch and continuous culture of animal cells Kadouri, A.; Spier, R. E. Cytotechnology, Vol. 24, Issue 2, p. 89-98 https://doi.org/10.1023/A:1007932614011	journal	July 1997
Functional genomic hypothesis generation and experimentation by a robot scientist King, Ross D.; Whelan, Kenneth E.; Jones, Ffion M. Nature, Vol. 427, Issue 6971 https://doi.org/10.1038/nature02236	journal	January 2004
Microfluidic Cell Retention Device for Perfusion of Mammalian Suspension Culture Kwon, Taehong; Prentice, Holly; Oliveira, Jonas De Scientific Reports, Vol. 7, Issue 1 https://doi.org/10.1038/s41598-017-06949-8	journal	July 2017
The microfluidic multitrap nanophysiometer for hematologic cancer cell characterization reveals temporal sensitivity of the calcein-AM efflux assay Byrd IV, Thomas F.; Hoang, Loi T.; Kim, Eric G. Scientific Reports, Vol. 4, Issue 1 https://doi.org/10.1038/srep05117	journal	May 2014
Membrane-less microfiltration using inertial microfluidics Warkiani, Majid Ebrahimi; Tay, Andy Kah Ping; Guan, Guofeng Scientific Reports, Vol. 5, Issue 1 https://doi.org/10.1038/srep11018	journal	July 2015
Slanted spiral microfluidics for the ultra-fast, label-free isolation of circulating tumor cells Warkiani, Majid Ebrahimi; Guan, Guofeng; Luan, Khoo Bee Lab Chip, Vol. 14, Issue 1 https://doi.org/10.1039/C3LC50617G	journal	January 2014
Broadscale resolving power performance of a high precision uniform field ion mobility-mass spectrometer May, Jody C.; Dodds, James N.; Kurulugama, Ruwan T. The Analyst, Vol. 140, Issue 20 https://doi.org/10.1039/C5AN00923E	journal	January 2015
Raman spectroscopy compatible PDMS droplet microfluidic culture and analysis platform towards on-chip lipidomics Kim, Hyun Soo; Waqued, Sergio C.; Nodurft, Dawson T. The Analyst, Vol. 142, Issue 7 https://doi.org/10.1039/C6AN02221A	journal	January 2017
Surface-enhanced Raman spectroscopy and microfluidic platforms: challenges, solutions and potential applications Jahn, I. J.; Žukovskaja, O.; Zheng, X. -S. The Analyst, Vol. 142, Issue 7 https://doi.org/10.1039/C7AN00118E	journal	January 2017
Integrating advanced functionality in a microfabricated high-throughput fluorescent-activated cell sorter Wolff, A.; Perch-Nielsen, I. R.; Larsen, U. D. Lab on a Chip, Vol. 3, Issue 1 https://doi.org/10.1039/b209333b	journal	January 2003
Integration of single cell injection, cell lysis, separation and detection of intracellular constituents on a microfluidic chipElectronic supplementary information (ESI) available: Video showing a single erythrocyte transported through the microchannel, docking (adhering) at a particular point and then being lysed. See http://www.rsc.org/suppdata/lc/b3/b310552k/ Gao, Jian; Yin, Xue-Feng; Fang, Zhao-Lun Lab on a Chip, Vol. 4, Issue 1 https://doi.org/10.1039/b310552k	journal	January 2004
Towards monitoring real-time cellular response using an integrated microfluidics-matrix assisted laser desorption ionisation/nanoelectrospray ionisation-ion mobility-mass spectrometry platform Enders, J. R.; Wikswo, J. P.; Seale, K. T. IET Systems Biology, Vol. 4, Issue 6 https://doi.org/10.1049/iet-syb.2010.0012	journal	November 2010
Recreating blood-brain barrier physiology and structure on chip: A novel neurovascular microfluidic bioreactor Brown, Jacquelyn A.; Pensabene, Virginia; Markov, Dmitry A. Biomicrofluidics, Vol. 9, Issue 5 https://doi.org/10.1063/1.4934713	journal	September 2015
Thermoplastic microfluidic bioreactors with integrated electrodes to study tumor treating fields on yeast cells Gencturk, Elif; Ulgen, Kutlu O.; Mutlu, Senol Biomicrofluidics, Vol. 14, Issue 3 https://doi.org/10.1063/5.0008462	journal	May 2020
Closed-loop cycles of experiment design, execution, and learning accelerate systems biology model development in yeast Coutant, Anthony; Roper, Katherine; Trejo-Banos, Daniel Proceedings of the National Academy of Sciences, Vol. 116, Issue 36 https://doi.org/10.1073/pnas.1900548116	journal	August 2019
Experiments with the Chemostat on Spontaneous Mutations of Bacteria Novick, A.; Szilard, L. Proceedings of the National Academy of Sciences, Vol. 36, Issue 12 https://doi.org/10.1073/pnas.36.12.708	journal	December 1950
Super-enhanced particle focusing in a novel microchannel geometry using inertial microfluidics Sonmez, U.; Jaber, S.; Trabzon, L. Journal of Micromechanics and Microengineering, Vol. 27, Issue 6 https://doi.org/10.1088/1361-6439/aa6b18	journal	April 2017
Cheaper faster drug development validated by the repositioning of drugs against neglected tropical diseases Williams, Kevin; Bilsland, Elizabeth; Sparkes, Andrew Journal of The Royal Society Interface, Vol. 12, Issue 104 https://doi.org/10.1098/rsif.2014.1289	journal	March 2015
Continuous culture - making a comeback? Hoskisson, P. A. Microbiology, Vol. 151, Issue 10 https://doi.org/10.1099/mic.0.27924-0	journal	October 2005
The Robot Scientist Adam King, Ross D.; Rowland, Jem; Aubrey, Wayne Computer, Vol. 42, Issue 7 https://doi.org/10.1109/MC.2009.270	journal	August 2009
Microfluidic Large-Scale Integration Thorsen, T. Science, Vol. 298, Issue 5593 https://doi.org/10.1126/science.1076996	journal	September 2002
Description of the Chemostat Novick, Aaron; Szilard, Leo Science, Vol. 112, Issue 2920 https://doi.org/10.1126/science.112.2920.715	journal	December 1950
The Automation of Science King, Ross D.; Rowland, Jem; Oliver, Stephen G. Science, Vol. 324, Issue 5923 https://doi.org/10.1126/science.1165620	journal	April 2009
Monolithic Microfabricated Valves and Pumps by Multilayer Soft Lithography Unger, M. A. Science, Vol. 288, Issue 5463 https://doi.org/10.1126/science.288.5463.113	journal	April 2000
Advanced Multidimensional Separations in Mass Spectrometry: Navigating the Big Data Deluge May, Jody C.; McLean, John A. Annual Review of Analytical Chemistry, Vol. 9, Issue 1 https://doi.org/10.1146/annurev-anchem-071015-041734	journal	June 2016
How Do Control-Based Approaches Enter into Biology? LeDuc, Philip R.; Messner, William C.; Wikswo, John P. Annual Review of Biomedical Engineering, Vol. 13, Issue 1 https://doi.org/10.1146/annurev-bioeng-071910-124651	journal	August 2011
Systems-level view of cocaine addiction: The interconnection of the immune and nervous systems Marasco, Christina C.; Goodwin, Cody R.; Winder, Danny G. Experimental Biology and Medicine, Vol. 239, Issue 11 https://doi.org/10.1177/1535370214537747	journal	June 2014
Fitting tissue chips and microphysiological systems into the grand scheme of medicine, biology, pharmacology, and toxicology Watson, David E.; Hunziker, Rosemarie; Wikswo, John P. Experimental Biology and Medicine, Vol. 242, Issue 16 https://doi.org/10.1177/1535370217732765	journal	June 2017
Circadian hormone control in a human-on-a-chip: In vitro biology’s ignored component? Cyr, Kevin J.; Avaldi, Omero M.; Wikswo, John P. Experimental Biology and Medicine, Vol. 242, Issue 17 https://doi.org/10.1177/1535370217732766	journal	October 2017
Study of a recombinant CHO cell line producing a monoclonal antibody by ATF or TFF external filter perfusion in a WAVE Bioreactor™ Clincke, Marie-Françoise; Mölleryd, Carin; Zhang, Ye BMC Proceedings, Vol. 5, Issue S8 https://doi.org/10.1186/1753-6561-5-S8-P105	journal	November 2011
Metabolic consequences of inflammatory disruption of the blood-brain barrier in an organ-on-chip model of the human neurovascular unit Brown, Jacquelyn A.; Codreanu, Simona G.; Shi, Mingjian Journal of Neuroinflammation, Vol. 13, Issue 1 https://doi.org/10.1186/s12974-016-0760-y	journal	December 2016
Advances in blood–brain barrier modeling in microphysiological systems highlight critical differences in opioid transport due to cortisol exposure Brown, Jacquelyn A.; Faley, Shannon L.; Shi, Yajuan Fluids and Barriers of the CNS, Vol. 17, Issue 1 https://doi.org/10.1186/s12987-020-00200-9	journal	June 2020
Real-Time Cellular Exometabolome Analysis with a Microfluidic-Mass Spectrometry Platform Marasco, Christina C.; Enders, Jeffrey R.; Seale, Kevin T. PLOS ONE, Vol. 10, Issue 2 https://doi.org/10.1371/journal.pone.0117685	journal	February 2015
Raman Spectroscopy Detects Biochemical Changes Due to Proliferation in Mammalian Cell Cultures Short, Kurt W.; Carpenter, Susan; Freyer, James P. Biophysical Journal, Vol. 88, Issue 6 https://doi.org/10.1529/biophysj.103.038604	journal	June 2005
Metabolic Discrimination of Select List Agents by Monitoring Cellular Responses in a Multianalyte Microphysiometer Eklund, Sven; Thompson, Roy; Snider, Rachel Sensors, Vol. 9, Issue 3 https://doi.org/10.3390/s90302117	journal	March 2009
The Use of Chemostats in Microbial Systems Biology Ziv, Naomi; Brandt, Nathan J.; Gresham, David Journal of Visualized Experiments, Issue 80 https://doi.org/10.3791/50168-v	journal	October 2013
An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects Ali, Ahmed; Abouleila, Yasmine; Germond, Arno Journal of Visualized Experiments, Issue 155 https://doi.org/10.3791/60449-v	journal	January 2020

Similar Records

Cartridge systems, capacitive pumps and multi-throw valves and pump-valve systems and applications of same

Patent · Tue Oct 05 00:00:00 EDT 2021 · OSTI ID:1859908

Cartridge systems, capacitive pumps and multi-throw valves and pump-valve systems and applications of same

Patent · Tue Oct 11 00:00:00 EDT 2022 · OSTI ID:1986675

Microfluidic systems, pumps, valves, fluidic chips thereof, and applications of same

Patent · Mon Jan 30 23:00:00 EST 2023 · OSTI ID:1987094

Massively parallel, multiple-organ perfusion control system

Citation Formats

References (62)

Similar Records

Related Subjects