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Production of fusel lactates via biocatalysis

Patent ·
OSTI ID:2222172

Microbial enzymes are used for esterification of biomass-derived substrates for production of industrially valuable esters. E. coli was used as an efficient platform for recombinant synthesis of fusel lactates such as the green solvent ethyl lactate.

Research Organization:
Sandia National Laboratories (SNL-CA), Livermore, CA (United States); Univ. of Arizona, Scottsdale, AZ (United States)
Sponsoring Organization:
USDOE National Nuclear Security Administration (NNSA)
DOE Contract Number:
NA0003525
Assignee:
National Technology & Engineering Solutions of Sandia, LLC (Albuquerque, NM); Arizona Board of Regents on behalf of Arizona State University (Scottsdale, AZ)
Patent Number(s):
11,725,220
Application Number:
17/003,061
OSTI ID:
2222172
Country of Publication:
United States
Language:
English

References (9)

Metabolic Engineering for Production of Biorenewable Fuels and Chemicals: Contributions of Synthetic Biology journal January 2010
Esterases of lactic acid bacteria and cheese flavour: Milk fat hydrolysis, alcoholysis and esterification journal June 2005
Ethyl lactate as a solvent: Properties, applications and production processes – a review journal January 2011
Accurately Determining Esterase Activity via the Isosbestic Point of p-Nitrophenol journal October 2016
Enzymes: principles and biotechnological applications journal October 2015
Engineered biosynthesis of medium-chain esters in Escherichia coli journal January 2015
Microbial biosynthesis of lactate esters journal September 2019
A novel process for the ethyl lactate synthesis in a simulated moving bed reactor (SMBR) journal July 2009
Ethyl lactate production via esterification of lactic acid with ethanol combined with pervaporation journal December 2010

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