Phase contrast tomography of the mouse cochlea at microfocus x-ray sources

Journal Article · Mon Aug 19 04:00:00 EDT 2013 · Applied Physics Letters

DOI:https://doi.org/10.1063/1.4818737· OSTI ID:22218138

Bartels, Matthias; Krenkel, Martin ^[1]; Hernandez, Victor H. ^[2]; Moser, Tobias ^[2]; Salditt, Tim ^[1]

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1. Institute for X-Ray Physics, University of Göttingen, Göttingen (Germany)
2. InnerEarLab, Department of Otolaryngology, University Medical Center Göttingen, Göttingen (Germany)

We present phase contrast x-ray tomography of functional soft tissue within the bony cochlear capsule of mice, carried out at laboratory microfocus sources with well-matched source, detector, geometry, and reconstruction algorithms at spatial resolutions down to 2 μm. Contrast, data quality and resolution enable the visualization of thin membranes and nerve fibers as well as automated segmentation of surrounding bone. By complementing synchrotron radiation imaging techniques, a broad range of biomedical applications becomes possible as demonstrated for optogenetic cochlear implant research.

• OSTI ID: 22218138
• Journal Information: Applied Physics Letters, Journal Name: Applied Physics Letters Journal Issue: 8 Vol. 103; ISSN APPLAB; ISSN 0003-6951
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
ALGORITHMS
ANIMAL TISSUES
AUDITORY ORGANS
CAPSULES
COMPUTERIZED TOMOGRAPHY
FIBERS
GEOMETRY
IMAGE PROCESSING
IMAGES
IMPLANTS
MEMBRANES
MICE
SKELETON
SPATIAL RESOLUTION
SYNCHROTRON RADIATION
X RADIATION
X-RAY SOURCES