Zac1, an Sp1-like protein, regulates human p21{sup WAF1/Cip1} gene expression in HeLa cells

Journal Article · Sat Dec 10 04:00:00 EST 2011 · Experimental Cell Research

DOI:https://doi.org/10.1016/J.YEXCR.2011.09.018· OSTI ID:22212258

Liu, Pei-Yao ^[1]; Hsieh, Tsai-Yuan ^[2]; Liu, Shu-Ting; Chang, Yung-Lung ^[3]; Lin, Wei-Shiang ^[4]; Wang, Wei-Ming ^[1]; Huang, Shih-Ming ^[1]

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1. Graduate Institute of Life Sciences, National Defense Medical Center, Taipei 114, Taiwan, ROC (China)
2. Division of Gastroenterology, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei 114, Taiwan, ROC (China)
3. Department of Biochemistry, National Defense Medical Center, Taipei 114, Taiwan, ROC (China)
4. Division of Cardiology, Department of Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei 114, Taiwan, ROC (China)

Zac1 functions as both a transcription factor and a transcriptional cofactor for p53, nuclear receptors (NRs) and NR coactivators. Zac1 might also act as a transcriptional repressor via the recruitment of histone deacetylase 1 (HDAC1). The ability of Zac1 to interact directly with GC-specific elements indicates that Zac1 possibly binds to Sp1-responsive elements. In the present study, our data show that Zac1 is able to interact directly with the Sp1-responsive element in the p21{sup WAF1/Cip1} gene promoter and enhance the transactivation activity of Sp1 through direct physical interaction. Our data further demonstrate that Zac1 might enhance Sp1-specific promoter activity by interacting with the Sp1-responsive element, affecting the transactivation activity of Sp1 via a protein-protein interaction, or competing the HDAC1 protein away from the pre-existing Sp1/HDAC1 complex. Finally, the synergistic regulation of p21{sup WAF1/Cip1} gene expression by Zac1 and Sp1 is mediated by endogenous p53 protein and p53-responsive elements in HeLa cells. Our work suggests that Zac1 might serve as an Sp1-like protein that directly interacts with the Sp1-responsive element to oligomerize with and/or to coactivate Sp1.

• OSTI ID: 22212258
• Journal Information: Experimental Cell Research, Journal Name: Experimental Cell Research Journal Issue: 20 Vol. 317; ISSN 0014-4827; ISSN ECREAL
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ADENOMAS
CELL CYCLE
GLUTATHIONE
HELA CELLS
RECEPTORS
TRANSCRIPTION FACTORS