Enhancement of neurite outgrowth in neuron cancer stem cells by growth on 3-D collagen scaffolds

Journal Article · Fri Nov 09 04:00:00 EST 2012 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2012.10.007· OSTI ID:22210321

Chen, Chih-Hao ^[1]; Kuo, Shyh Ming ^[2]; Liu, Guei-Sheung ^[3]; Chen, Wan-Nan U. ^[4]; Chuang, Chin-Wen ^[1]; Liu, Li-Feng ^[4]

---

1. Department of Electrical Engineering, I-Shou University, Taiwan, ROC (China)
2. Department of Biomedical Engineering, I-Shou University, Taiwan, ROC (China)
3. Centre for Eye Research Australia, University of Melbourne (Australia)
4. Department of Biological Science and Technology, I-Shou University, Taiwan, ROC (China)

Highlights: Black-Right-Pointing-Pointer Neuron cancer stem cells (NCSCs) behave high multiply of growth on collagen scaffold. Black-Right-Pointing-Pointer Enhancement of NCSCs neurite outgrowth on porous collagen scaffold. Black-Right-Pointing-Pointer 3-D collagen culture of NCSCs shows an advance differentiation than 2-D culture. -- Abstract: Collagen is one component of the extracellular matrix that has been widely used for constructive remodeling to facilitate cell growth and differentiation. The 3-D distribution and growth of cells within the porous scaffold suggest a clinical significance for nerve tissue engineering. In the current study, we investigated proliferation and differentiation of neuron cancer stem cells (NCSCs) on a 3-D porous collagen scaffold that mimics the natural extracellular matrix. We first generated green fluorescence protein (GFP) expressing NCSCs using a lentiviral system to instantly monitor the transitions of morphological changes during growth on the 3-D scaffold. We found that proliferation of GFP-NCSCs increased, and a single cell mass rapidly grew with unrestricted expansion between days 3 and 9 in culture. Moreover, immunostaining with neuronal nuclei (NeuN) revealed that NCSCs grown on the 3-D collagen scaffold significantly enhanced neurite outgrowth. Our findings confirmed that the 80 {mu}m porous collagen scaffold could enhance attachment, viability and differentiation of the cancer neural stem cells. This result could provide a new application for nerve tissue engineering and nerve regeneration.

• OSTI ID: 22210321
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1 Vol. 428; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CELL PROLIFERATION
COLLAGEN
FLUORESCENCE
MORPHOLOGICAL CHANGES
NEOPLASMS
NERVE CELLS
NERVE TISSUE
POROUS MATERIALS
REGENERATION
STEM CELLS