Inhibition of NAMPT pathway by FK866 activates the function of p53 in HEK293T cells

Journal Article · Fri Aug 03 04:00:00 EDT 2012 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2012.06.075· OSTI ID:22210161

Dittrich, Tino ^[1]; Chandra, Prakash ^[2]; Becker, Annette ^[3]; Lippka, Yannick ^[4]; Selvakumar, Divakarvel ^[4]; Klusmann, Jan-Henning; Reinhardt, Dirk ^[1]; Welte, Karl ^[4]

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1. Department of Pediatrics, Hematology and Oncology, Hannover Medical School, Carl Neuberg Str-1, 30625 Hannover (Germany)
2. Frankfurt University Medical School, Molecular Biology, Building 57, Theodor-Stern-Kai-7, 60590 Frankfurt (Germany)
3. Department of Biology, Technical University of Darmstadt, 64287 Darmstadt (Germany)
4. Department of Molecular Hematopoiesis, Hannover Medical School, Carl Neuberg Str-1, 30625 Hannover (Germany)

Highlights: Black-Right-Pointing-Pointer In 293T cells, p53 is considered to be inactive due to its interaction with the large T-antigen. Black-Right-Pointing-Pointer Acetylation of p53 at lysine 382 is important for its functional activation. Black-Right-Pointing-Pointer First evidence to document the presence of a functional p53 in 293T cells. Black-Right-Pointing-Pointer Inhibition of NAMPT/SIRT pathway by FK866 in 293T cells increases the functional activity of p53. Black-Right-Pointing-Pointer This activation of p53 involves reversible acetylation of p53 at lysine 382. -- Abstract: Inactivation of p53 protein by endogenous and exogenous carcinogens is involved in the pathogenesis of different human malignancies. In cancer associated with SV-40 DNA tumor virus, p53 is considered to be non-functional mainly due to its interaction with the large T-antigen. Using the 293T cell line (HEK293 cells transformed with large T antigen) as a model, we provide evidence that p53 is one of the critical downstream targets involved in FK866-mediated killing of 293T cells. A reduced rate of apoptosis and an increased number of cells in S-phase was accompanied after knockdown of p53 in these cells. Inhibition of NAMPT by FK866, or inhibition of SIRT by nicotinamide decreased proliferation and triggered death of 293T cells involving the p53 acetylation pathway. Additionally, knockdown of p53 attenuated the effect of FK866 on cell proliferation, apoptosis, and cell cycle arrest. The data presented here shed light on two important facts: (1) that p53 in 293T cells is active in the presence of FK866, an inhibitor of NAMPT pathway; (2) the apoptosis induced by FK866 in 293T cells is associated with increased acetylation of p53 at Lys382, which is required for the functional activity of p53.

• OSTI ID: 22210161
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 3 Vol. 424; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ACETYLATION
ANTIGENS
APOPTOSIS
CARCINOGENS
CELL CYCLE
CELL PROLIFERATION
DEATH
DNA
INACTIVATION
INHIBITION
LYSINE
NEOPLASMS
NICOTINAMIDE
ONCOGENIC VIRUSES
PATHOGENESIS
PROTEINS