Neuropilin2 expressed in gastric cancer endothelial cells increases the proliferation and migration of endothelial cells in response to VEGF

Journal Article · Sat Aug 01 04:00:00 EDT 2009 · Experimental Cell Research

DOI:https://doi.org/10.1016/J.YEXCR.2009.04.018· OSTI ID:22209784

Kim, Woo Ho ^[1]; Lee, Sun Hee; Jung, Myung Hwan ^[2]; Seo, Ji Heun; Kim, Jin; Kim, Min A ^[1]

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1. Department of Pathology and Surgery, Cancer Research Institute, Seoul National University College of Medicine, Seoul, 110-799 (Korea, Republic of)
2. School of Life Sciences and Biotechnology, Department of Natural Sciences, Kyungpook National University, Daegu, 702-701 (Korea, Republic of)

The structure and characteristics of the tumor vasculature are known to be different from those of normal vessels. Neuropilin2 (Nrp2), which is expressed in non-endothelial cell types, such as neuronal or cancer cells, functions as a receptor for both semaphorin and vascular endothelial growth factor (VEGF). After isolating tumor and normal endothelial cells from advanced gastric cancer tissue and normal gastric mucosa tissues, respectively, we identified genes that were differentially expressed in gastric tumor endothelial (TEC) and normal endothelial cells (NEC) using DNA oligomer chips. Using reverse transcriptase-PCR, we confirmed the chip results by showing that Nrp2 gene expression is significantly up-regulated in TEC. Genes that were found to be up-regulated in TEC were also observed to be up-regulated in human umbilical vein endothelial cells (HUVECs) that were co-cultured with gastric cancer cells. In addition, HUVECs co-cultured with gastric cancer cells showed an increased reactivity to VEGF-induced proliferation and migration. Moreover, overexpression of Nrp2 in HUVECs significantly enhanced the proliferation and migration induced by VEGF. Observation of an immunohistochemical analysis of various human tumor tissue arrays revealed that Nrp2 is highly expressed in the tumor vessel lining and to a lesser extent in normal tissue microvessels. From these results, we suggest that Nrp2 may function to increase the response to VEGF, which is more significant in TEC than in NEC given the differential expression, leading to gastric TEC with aggressive angiogenesis phenotypes.

• OSTI ID: 22209784
• Journal Information: Experimental Cell Research, Journal Name: Experimental Cell Research Journal Issue: 13 Vol. 315; ISSN 0014-4827; ISSN ECREAL
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ANGIOGENESIS
ANIMAL TISSUES
CELL PROLIFERATION
DNA
GENES
GROWTH FACTORS
MUCOUS MEMBRANES
NEOPLASMS
PHENOTYPE
POLYMERASE CHAIN REACTION
RECEPTORS
VEINS