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Title: Ryanodine receptors are involved in nuclear calcium oscillation in primary pancreatic {beta}-cells

Abstract

Highlights: Black-Right-Pointing-Pointer We found that RyRs are expressed on the nuclear envelope in single primary pancreatic {beta}-cells and isolated nuclei. Black-Right-Pointing-Pointer We showed that the pattern of glucose-induced Ca{sup 2+} oscillation in the nucleus and cytosol was similar. Black-Right-Pointing-Pointer Our results demonstrate that ryanodine-sensitive Ca{sup 2+} stores exist and have function in the pancreatic {beta}-cell nucleus. -- Abstract: Ryanodine receptors (RyRs) are mainly located on the endoplasmic reticulum (ER) and play an important role in regulating glucose-induced cytosolic Ca{sup 2+} oscillation in pancreatic {beta}-cells. However, subcellular locations and functions of RyRs on other cell organelles such as nuclear envelope are not well understood. In order to investigate the role of RyRs in nuclear Ca{sup 2+} oscillation we designed and conducted experiments in intact primary pancreatic {beta}-cells. Immunocytochemistry was used to examine the expression of RYRs on the nuclear envelope. Confocal microscopy was used to evaluate the function of RYRs on the nuclear envelope. We found that RyRs are expressed on the nuclear envelope in single primary pancreatic {beta}-cells and isolated nuclei. Laser scanning confocal microscopy studies indicated that application of glucose to the cells co-incubated with Ca{sup 2+} indicator Fluo-4 AM and cell-permeable nuclear indicator Hoechst 33342 resulted in nuclearmore » Ca{sup 2+} oscillation. The pattern of glucose-induced Ca{sup 2+} oscillation in the nucleus and cytosol was similar. The reduction of Ca{sup 2+} oscillation amplitude by ryanodine was much greater in the nucleus though both the cytosol and the nucleus Ca{sup 2+} amplitude decreased by ryanodine. Our results suggest that functional ryanodine receptors not only exist in endoplasmic reticulum but are also expressed in nuclear envelope of pancreatic {beta}-cells.« less

Authors:
 [1];  [2];  [3];  [4]; ;  [3];  [1];  [3]
  1. Urological Surgery Research Institute, Southwest Hospital, Third Military Medical University, Chongqing (China)
  2. (China)
  3. National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing (China)
  4. (United States)
Publication Date:
OSTI Identifier:
22207911
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 423; Journal Issue: 2; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; AMPLITUDES; CALCIUM; CALCIUM IONS; CELL NUCLEI; ENDOPLASMIC RETICULUM; GLUCOSE; MICROSCOPY; PANCREAS; RECEPTORS

Citation Formats

Zheng, Ji, National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, Chen, Zheng, Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI, Yin, Wenxuan, Miao, Lin, Zhou, Zhansong, E-mail: Zhouzhansong@sohu.com, and Ji, Guangju, E-mail: Gj28@ibp.ac.cn. Ryanodine receptors are involved in nuclear calcium oscillation in primary pancreatic {beta}-cells. United States: N. p., 2012. Web. doi:10.1016/J.BBRC.2012.04.114.
Zheng, Ji, National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, Chen, Zheng, Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI, Yin, Wenxuan, Miao, Lin, Zhou, Zhansong, E-mail: Zhouzhansong@sohu.com, & Ji, Guangju, E-mail: Gj28@ibp.ac.cn. Ryanodine receptors are involved in nuclear calcium oscillation in primary pancreatic {beta}-cells. United States. doi:10.1016/J.BBRC.2012.04.114.
Zheng, Ji, National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, Chen, Zheng, Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI, Yin, Wenxuan, Miao, Lin, Zhou, Zhansong, E-mail: Zhouzhansong@sohu.com, and Ji, Guangju, E-mail: Gj28@ibp.ac.cn. Fri . "Ryanodine receptors are involved in nuclear calcium oscillation in primary pancreatic {beta}-cells". United States. doi:10.1016/J.BBRC.2012.04.114.
@article{osti_22207911,
title = {Ryanodine receptors are involved in nuclear calcium oscillation in primary pancreatic {beta}-cells},
author = {Zheng, Ji and National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing and Chen, Zheng and Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI and Yin, Wenxuan and Miao, Lin and Zhou, Zhansong, E-mail: Zhouzhansong@sohu.com and Ji, Guangju, E-mail: Gj28@ibp.ac.cn},
abstractNote = {Highlights: Black-Right-Pointing-Pointer We found that RyRs are expressed on the nuclear envelope in single primary pancreatic {beta}-cells and isolated nuclei. Black-Right-Pointing-Pointer We showed that the pattern of glucose-induced Ca{sup 2+} oscillation in the nucleus and cytosol was similar. Black-Right-Pointing-Pointer Our results demonstrate that ryanodine-sensitive Ca{sup 2+} stores exist and have function in the pancreatic {beta}-cell nucleus. -- Abstract: Ryanodine receptors (RyRs) are mainly located on the endoplasmic reticulum (ER) and play an important role in regulating glucose-induced cytosolic Ca{sup 2+} oscillation in pancreatic {beta}-cells. However, subcellular locations and functions of RyRs on other cell organelles such as nuclear envelope are not well understood. In order to investigate the role of RyRs in nuclear Ca{sup 2+} oscillation we designed and conducted experiments in intact primary pancreatic {beta}-cells. Immunocytochemistry was used to examine the expression of RYRs on the nuclear envelope. Confocal microscopy was used to evaluate the function of RYRs on the nuclear envelope. We found that RyRs are expressed on the nuclear envelope in single primary pancreatic {beta}-cells and isolated nuclei. Laser scanning confocal microscopy studies indicated that application of glucose to the cells co-incubated with Ca{sup 2+} indicator Fluo-4 AM and cell-permeable nuclear indicator Hoechst 33342 resulted in nuclear Ca{sup 2+} oscillation. The pattern of glucose-induced Ca{sup 2+} oscillation in the nucleus and cytosol was similar. The reduction of Ca{sup 2+} oscillation amplitude by ryanodine was much greater in the nucleus though both the cytosol and the nucleus Ca{sup 2+} amplitude decreased by ryanodine. Our results suggest that functional ryanodine receptors not only exist in endoplasmic reticulum but are also expressed in nuclear envelope of pancreatic {beta}-cells.},
doi = {10.1016/J.BBRC.2012.04.114},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 2,
volume = 423,
place = {United States},
year = {2012},
month = {6}
}