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Title: The combination of lanthanum chloride and the calcimimetic calindol delays the progression of vascular smooth muscle cells calcification

Abstract

Highlights: Black-Right-Pointing-Pointer Lanthanum reduces the progression of high phosphate-induced calcium deposition. Black-Right-Pointing-Pointer Calcium receptor agonists and the calcimimetic calindol reduce calcium deposition. Black-Right-Pointing-Pointer Lanthanum and calindol cooperate on reducing calcium deposition. Black-Right-Pointing-Pointer Lanthanum and calindol may interact with the same receptor. -- Abstract: Phosphate (Pi)-binders are commonly used in dialysis patients to control high Pi levels, that associated with vascular calcification (VC). The aim of this study was to investigate the effects of lanthanum chloride (LaCl{sub 3}) on the progression of high Pi-induced VC, in rat vascular smooth muscle cells (VSMCs). Pi-induced Ca deposition was inhibited by LaCl{sub 3}, with a maximal effect at 100 {mu}M (59.0 {+-} 2.5% inhibition). Furthermore, we studied the effects on VC of calcium sensing receptor (CaSR) agonists. Gadolinium chloride, neomycin, spermine, and the calcimimetic calindol significantly inhibited Pi-induced VC (55.9 {+-} 2.2%, 37.3 {+-} 4.7%, 30.2 {+-} 5.7%, and 63.8 {+-} 5.7%, respectively). To investigate the hypothesis that LaCl{sub 3} reduces the progression of VC by interacting with the CaSR, we performed a concentration-response curve of LaCl{sub 3} in presence of a sub-effective concentration of calindol (10 nM). Interestingly, this curve was shifted to the left (IC{sub 50} 9.6 {+-} 2.6 {mu}M), compared tomore » the curve in the presence of LaCl{sub 3} alone (IC{sub 50} 19.0 {+-} 4.8 {mu}M). In conclusion, we demonstrated that lanthanum chloride effectively reduces the progression of high phosphate-induced vascular calcification. In addition, LaCl{sub 3} cooperates with the calcimimetic calindol in decreasing Ca deposition in this in vitro model. These results suggest the potential role of lanthanum in the treatment of VC induced by high Pi.« less

Authors:
; ; ;  [1];  [2];  [1];  [1]
  1. Renal Division and Laboratory of Experimental Nephrology, Dipartimento di Medicina e Chirurgia, Universita di Milano, Milan (Italy)
  2. Dipartimento di Salute Pubblica, Microbiologia e Virologia, Universita di Milano, Milan (Italy)
Publication Date:
OSTI Identifier:
22207723
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 418; Journal Issue: 4; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ABUNDANCE; BIOLOGICAL ACCUMULATION; BIOLOGICAL LOCALIZATION; DIALYSIS; GADOLINIUM CHLORIDES; IN VITRO; LANTHANUM CHLORIDES; MUSCLES; NEOMYCIN; PHOSPHATES; RATS; RECEPTORS; SPERMINE; VANADIUM CARBIDES

Citation Formats

Ciceri, Paola, Volpi, Elisa, Brenna, Irene, Elli, Francesca, Borghi, Elisa, Brancaccio, Diego, and Cozzolino, Mario, E-mail: mario.cozzolino@unimi.it. The combination of lanthanum chloride and the calcimimetic calindol delays the progression of vascular smooth muscle cells calcification. United States: N. p., 2012. Web. doi:10.1016/J.BBRC.2012.01.097.
Ciceri, Paola, Volpi, Elisa, Brenna, Irene, Elli, Francesca, Borghi, Elisa, Brancaccio, Diego, & Cozzolino, Mario, E-mail: mario.cozzolino@unimi.it. The combination of lanthanum chloride and the calcimimetic calindol delays the progression of vascular smooth muscle cells calcification. United States. doi:10.1016/J.BBRC.2012.01.097.
Ciceri, Paola, Volpi, Elisa, Brenna, Irene, Elli, Francesca, Borghi, Elisa, Brancaccio, Diego, and Cozzolino, Mario, E-mail: mario.cozzolino@unimi.it. Fri . "The combination of lanthanum chloride and the calcimimetic calindol delays the progression of vascular smooth muscle cells calcification". United States. doi:10.1016/J.BBRC.2012.01.097.
@article{osti_22207723,
title = {The combination of lanthanum chloride and the calcimimetic calindol delays the progression of vascular smooth muscle cells calcification},
author = {Ciceri, Paola and Volpi, Elisa and Brenna, Irene and Elli, Francesca and Borghi, Elisa and Brancaccio, Diego and Cozzolino, Mario, E-mail: mario.cozzolino@unimi.it},
abstractNote = {Highlights: Black-Right-Pointing-Pointer Lanthanum reduces the progression of high phosphate-induced calcium deposition. Black-Right-Pointing-Pointer Calcium receptor agonists and the calcimimetic calindol reduce calcium deposition. Black-Right-Pointing-Pointer Lanthanum and calindol cooperate on reducing calcium deposition. Black-Right-Pointing-Pointer Lanthanum and calindol may interact with the same receptor. -- Abstract: Phosphate (Pi)-binders are commonly used in dialysis patients to control high Pi levels, that associated with vascular calcification (VC). The aim of this study was to investigate the effects of lanthanum chloride (LaCl{sub 3}) on the progression of high Pi-induced VC, in rat vascular smooth muscle cells (VSMCs). Pi-induced Ca deposition was inhibited by LaCl{sub 3}, with a maximal effect at 100 {mu}M (59.0 {+-} 2.5% inhibition). Furthermore, we studied the effects on VC of calcium sensing receptor (CaSR) agonists. Gadolinium chloride, neomycin, spermine, and the calcimimetic calindol significantly inhibited Pi-induced VC (55.9 {+-} 2.2%, 37.3 {+-} 4.7%, 30.2 {+-} 5.7%, and 63.8 {+-} 5.7%, respectively). To investigate the hypothesis that LaCl{sub 3} reduces the progression of VC by interacting with the CaSR, we performed a concentration-response curve of LaCl{sub 3} in presence of a sub-effective concentration of calindol (10 nM). Interestingly, this curve was shifted to the left (IC{sub 50} 9.6 {+-} 2.6 {mu}M), compared to the curve in the presence of LaCl{sub 3} alone (IC{sub 50} 19.0 {+-} 4.8 {mu}M). In conclusion, we demonstrated that lanthanum chloride effectively reduces the progression of high phosphate-induced vascular calcification. In addition, LaCl{sub 3} cooperates with the calcimimetic calindol in decreasing Ca deposition in this in vitro model. These results suggest the potential role of lanthanum in the treatment of VC induced by high Pi.},
doi = {10.1016/J.BBRC.2012.01.097},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 418,
place = {United States},
year = {Fri Feb 24 00:00:00 EST 2012},
month = {Fri Feb 24 00:00:00 EST 2012}
}