skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Cancer stem cell-like cells from a single cell of oral squamous carcinoma cell lines

Abstract

Research highlights: {yields} Four oral squamous cancer cell lines (OSCCL) were analyzed for cancer stem cells (CSCs). {yields} Single cell derived colonies of OSCCL express CSC-marker CD133 differentially. {yields} Monoclonal cell lines showed reduced sensitivity for Paclitaxel. {yields} In situ CD133{sup +} cells are slow cycling (Ki67-) indicating a reduced drug sensitivity. {yields} CD133{sup +} and CSC-like cells can be obtained from single colony forming cells of OSCCL. -- Abstract: Resistance of oral squamous cell carcinomas (OSCC) to conventional chemotherapy or radiation therapy might be due to cancer stem cells (CSCs). The development of novel anticancer drugs requires a simple method for the enrichment of CSCs. CSCs can be enriched from OSCC cell lines, for example, after cultivation in serum-free cell culture medium (SFM). In our study, we analyzed four OSCC cell lines for the presence of CSCs. CSC-like cells could not be enriched with SFM. However, cell lines obtained from holoclone colonies showed CSC-like properties such as a reduced rate of cell proliferation and a reduced sensitivity to Paclitaxel in comparison to cells from the parental lineage. Moreover, these cell lines differentially expressed the CSC-marker CD133, which is also upregulated in OSCC tissues. Interestingly, CD133{sup +} cells in OSCCmore » tissues expressed little to no Ki67, the cell proliferation marker that also indicates reduced drug sensitivity. Our study shows a method for the isolation of CSC-like cell lines from OSCC cell lines. These CSC-like cell lines could be new targets for the development of anticancer drugs under in vitro conditions.« less

Authors:
 [1];  [2]; ; ;  [3];  [4];  [3];  [5];  [6];  [3];  [1];  [1]
  1. Department of Operative Dentistry and Periodontology, University of Regensburg (Germany)
  2. (Germany)
  3. Department of Oral and Maxillofacial Surgery, University of Regensburg (Germany)
  4. Department of Gynecology and Obstetrics, University of Regensburg (Germany)
  5. Institute of Pathology, University of Regensburg (Germany)
  6. Department of Radiotherapy, University of Regensburg (Germany)
Publication Date:
OSTI Identifier:
22204859
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 407; Journal Issue: 1; Other Information: Copyright (c) 2011 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; ANTINEOPLASTIC DRUGS; CARCINOMAS; CELL CULTURES; CELL PROLIFERATION; CHEMOTHERAPY; CULTIVATION; IN VITRO; RADIOSENSITIVITY; RADIOTHERAPY; STEM CELLS

Citation Formats

Felthaus, O., Department of Oral and Maxillofacial Surgery, University of Regensburg, Ettl, T., Gosau, M., Driemel, O., Brockhoff, G., Reck, A., Zeitler, K., Hautmann, M., Reichert, T.E., Schmalz, G., and Morsczeck, C., E-mail: christian.morsczeck@klinik.uni-regensburg.de. Cancer stem cell-like cells from a single cell of oral squamous carcinoma cell lines. United States: N. p., 2011. Web. doi:10.1016/J.BBRC.2011.02.084.
Felthaus, O., Department of Oral and Maxillofacial Surgery, University of Regensburg, Ettl, T., Gosau, M., Driemel, O., Brockhoff, G., Reck, A., Zeitler, K., Hautmann, M., Reichert, T.E., Schmalz, G., & Morsczeck, C., E-mail: christian.morsczeck@klinik.uni-regensburg.de. Cancer stem cell-like cells from a single cell of oral squamous carcinoma cell lines. United States. doi:10.1016/J.BBRC.2011.02.084.
Felthaus, O., Department of Oral and Maxillofacial Surgery, University of Regensburg, Ettl, T., Gosau, M., Driemel, O., Brockhoff, G., Reck, A., Zeitler, K., Hautmann, M., Reichert, T.E., Schmalz, G., and Morsczeck, C., E-mail: christian.morsczeck@klinik.uni-regensburg.de. Fri . "Cancer stem cell-like cells from a single cell of oral squamous carcinoma cell lines". United States. doi:10.1016/J.BBRC.2011.02.084.
@article{osti_22204859,
title = {Cancer stem cell-like cells from a single cell of oral squamous carcinoma cell lines},
author = {Felthaus, O. and Department of Oral and Maxillofacial Surgery, University of Regensburg and Ettl, T. and Gosau, M. and Driemel, O. and Brockhoff, G. and Reck, A. and Zeitler, K. and Hautmann, M. and Reichert, T.E. and Schmalz, G. and Morsczeck, C., E-mail: christian.morsczeck@klinik.uni-regensburg.de},
abstractNote = {Research highlights: {yields} Four oral squamous cancer cell lines (OSCCL) were analyzed for cancer stem cells (CSCs). {yields} Single cell derived colonies of OSCCL express CSC-marker CD133 differentially. {yields} Monoclonal cell lines showed reduced sensitivity for Paclitaxel. {yields} In situ CD133{sup +} cells are slow cycling (Ki67-) indicating a reduced drug sensitivity. {yields} CD133{sup +} and CSC-like cells can be obtained from single colony forming cells of OSCCL. -- Abstract: Resistance of oral squamous cell carcinomas (OSCC) to conventional chemotherapy or radiation therapy might be due to cancer stem cells (CSCs). The development of novel anticancer drugs requires a simple method for the enrichment of CSCs. CSCs can be enriched from OSCC cell lines, for example, after cultivation in serum-free cell culture medium (SFM). In our study, we analyzed four OSCC cell lines for the presence of CSCs. CSC-like cells could not be enriched with SFM. However, cell lines obtained from holoclone colonies showed CSC-like properties such as a reduced rate of cell proliferation and a reduced sensitivity to Paclitaxel in comparison to cells from the parental lineage. Moreover, these cell lines differentially expressed the CSC-marker CD133, which is also upregulated in OSCC tissues. Interestingly, CD133{sup +} cells in OSCC tissues expressed little to no Ki67, the cell proliferation marker that also indicates reduced drug sensitivity. Our study shows a method for the isolation of CSC-like cell lines from OSCC cell lines. These CSC-like cell lines could be new targets for the development of anticancer drugs under in vitro conditions.},
doi = {10.1016/J.BBRC.2011.02.084},
journal = {Biochemical and Biophysical Research Communications},
number = 1,
volume = 407,
place = {United States},
year = {Fri Apr 01 00:00:00 EDT 2011},
month = {Fri Apr 01 00:00:00 EDT 2011}
}