Liver X Receptor (LXR) activation negatively regulates visfatin expression in macrophages

Journal Article · Fri Jan 07 04:00:00 UTC 2011 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2010.12.002· OSTI ID:22204744

Mayi, Therese Hervee; Rigamonti, Elena ^[1]; INSERM UR1011, F-59000 Lille ^[2]; UDSL, F-59000 Lille ^[2]; Pattou, Francois ^[1]; Staels, Bart ^[1]; INSERM UR1011, F-59000 Lille ^[2]; UDSL, F-59000 Lille ^[2]; Chinetti-Gbaguidi, Giulia ^[1]; INSERM UR1011, F-59000 Lille ^[2]; UDSL, F-59000 Lille ^[2]

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1. Univ Lille Nord de France, F-59000 Lille (France)
2. France

Research highlights: {yields} Synthetic LXR ligands decreased visfatin expression in human macrophages. {yields} LXR activation leads to a modest and transient decrease of NAD{sup +} concentration. {yields} LXR activation decreased PPAR{gamma}-induced visfatin in human macrophages. -- Abstract: Adipose tissue macrophages (ATM) are the major source of visfatin, a visceral fat adipokine upregulated during obesity. Also known to play a role in B cell differentiation (pre-B cell colony-enhancing factor (PBEF)) and NAD biosynthesis (nicotinamide phosphoribosyl transferase (NAMPT)), visfatin has been suggested to play a role in inflammation. Liver X Receptor (LXR) and Peroxisome Proliferator-Activated Receptor (PPAR){gamma} are nuclear receptors expressed in macrophages controlling the inflammatory response. Recently, we reported visfatin as a PPAR{gamma} target gene in human macrophages. In this study, we examined whether LXR regulates macrophage visfatin expression. Synthetic LXR ligands decreased visfatin gene expression in a LXR-dependent manner in human and murine macrophages. The decrease of visfatin mRNA was paralleled by a decrease of protein secretion. Consequently, a modest and transient decrease of NAD{sup +} concentration was observed. Interestingly, LXR activation decreased the PPAR{gamma}-induced visfatin gene and protein secretion in human macrophages. Our results identify visfatin as a gene oppositely regulated by the LXR and PPAR{gamma} pathways in human macrophages.

• OSTI ID: 22204744
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1 Vol. 404; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ADIPOSE TISSUE
BIOSYNTHESIS
CELL DIFFERENTIATION
CONCENTRATION RATIO
GENES
INFLAMMATION
LIGANDS
LIVER
MACROPHAGES
MESSENGER-RNA
METABOLIC DISEASES
NAD
NICOTINAMIDE
RECEPTORS
SECRETION