ADAM15 expression is downregulated in melanoma metastasis compared to primary melanoma

Journal Article · Fri Oct 22 04:00:00 EDT 2010 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2010.09.055· OSTI ID:22202817

Ungerer, Christopher; Doberstein, Kai ^[1]; Buerger, Claudia; Hardt, Katja; Boehncke, Wolf-Henning ^[2]; Boehm, Beate ^[3]; Pfeilschifter, Josef ^[1]; Dummer, Reinhard ^[4]; Mihic-Probst, Daniela ^[5]; Gutwein, Paul ^[1]

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1. Pharmazentrum Frankfurt/ZAFES, University Hospital Goethe University Frankfurt, Frankfurt am Main (Germany)
2. Department of Dermatology, Clinic of the Goethe-University, Theodor-Stern-Kai, Frankfurt (Germany)
3. Division of Rheumatology, Goethe University, Frankfurt am Main (Germany)
4. Department of Pathology, Institute of Surgical Pathology, University Hospital, Zurich (Switzerland)
5. Department of Dermatology, University Hospital Zurich (Switzerland)

Research highlights: {yields} Strong ADAM15 expression is found in normal melanocytes. {yields} ADAM15 expression is significantly downregulated in patients with melanoma metastasis. {yields} TGF-{beta} can downregulate ADAM15 expression in melanoma cells. {yields} Overexpression of ADAM15 in melanoma cells inhibits migration, proliferation and invasion of melanoma cells. {yields} Conclusion: ADAM15 represents an tumor suppressor protein in melanoma. -- Abstract: In a mouse melanoma metastasis model it has been recently shown that ADAM15 overexpression in melanoma cells significantly reduced the number of metastatic nodules on the lung. Unfortunately, the expression of ADAM15 in human melanoma tissue has not been determined so far. In our study, we characterized the expression of ADAM15 in tissue micro-arrays of patients with primary melanoma with melanoma metastasis. ADAM15 was expressed in melanocytes and endothelial cells of benign nevi and melanoma tissue. Importantly, ADAM15 was significantly downregulated in melanoma metastasis compared to primary melanoma. We further demonstrate that IFN-{gamma} and TGF-{beta} downregulate ADAM15 protein levels in melanoma cells. To investigate the role of ADAM15 in melanoma progression, we overexpressed ADAM15 in melanoma cells. Importantly, overexpression of ADAM15 in melanoma cells reduced the migration, invasion and the anchorage dependent and independent cell growth of melanoma cells. In summary, the downregulation of ADAM15 plays an important role in melanoma progression and ADAM15 act as a tumorsuppressor in melanoma.

• OSTI ID: 22202817
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 3 Vol. 401; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
CELL PROLIFERATION
LUNGS
MELANIN
MELANOMAS
METASTASES
MICE
PATIENTS
PROTEINS