{beta}-Catenin/LEF1 activated enamelin expression in ameloblast-like cells

Journal Article · Fri Jul 30 00:00:00 EDT 2010 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2010.06.111· OSTI ID:22202717

Tian, Hua; Lv, Ping ^[1]; Ma, Kangtao ^[2]; Zhou, Chunyan ^[2]; Gao, Xuejun ^[1]

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1. Department of Cariology and Endodontology, Peking University School and Hospital of Stomatology, Beijing 100081 (China)
2. Department of Biochemistry and Molecular Biology, Peking University School of Basic Medical Sciences, Beijing 100191 (China)

Research highlights: {yields} {beta}-Catenin/LEF1 complex could activate enamelin gene transcription. {yields} {beta}-Catenin/LEF1 can directly bind to enamelin 5' regulatory region. {yields} Wnt/{beta}-catenin signaling can upregulate enamelin expression in ameloblast-like cells. -- Abstract: Enamelin is an ameloblast-specific matrix protein believed to play essential roles in enamel formation. However, mechanisms of enamelin transcription regulation are not clear. {beta}-Catenin/LEF1 is a key transcriptional complex involved in tooth development. In this study, the role of {beta}-catenin/LEF1 in enamelin expression was investigated. The 5'-flanking region of the mouse enamelin gene was analyzed and cloned. Co-transfection analysis and mutation assays revealed that two conserved LEF1 responsive elements located at -1002 and -597 bp upstream of the enamelin translation initiation site could augment transcriptional activity of the enamelin. The interaction between the enamelin elements and {beta}-catenin/LEF1 was further confirmed by electrophoresis mobility shift assays and chromatin immunoprecipitation assays. In addition, LiCl treatment induced nuclear translocation of {beta}-catenin and elevated endogenous enamelin expression in mouse ameloblast-like cells. The results suggested that Wnt/{beta}-catenin signaling could function in enamelin gene expression by direct interaction through two conserved LEF1 responsive elements on the enamelin gene in ameloblast-like cells.

• OSTI ID: 22202717
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 398, Issue 3; Other Information: Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CHROMATIN
ELECTROPHORESIS
ENAMELS
GENE REGULATION
GENES
LITHIUM CHLORIDES
MICE
MUTATIONS
PROTEINS
TEETH
TRANSCRIPTION
TRANSLOCATION