Involvement of the endocannabinoid system in periodontal healing

Journal Article · Fri Apr 16 04:00:00 EDT 2010 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2010.03.080· OSTI ID:22202475

Kozono, Sayaka ^[1]; Matsuyama, Takashi ^[1]; Biwasa, Kamal Krishna ^[2]; Kawahara, Ko-ichi ^[3]; Nakajima, Yumiko; Yoshimoto, Takehiko; Yonamine, Yutaka; Kadomatsu, Hideshi ^[1]; Tancharoen, Salunya ^[4]; Hashiguchi, Teruto ^[3]; Noguchi, Kazuyuki ^[1]; Maruyama, Ikuro ^[3]

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1. Department of Periodontology, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8520 (Japan)
2. Department of Biochemistry and Molecular Biology, Rajshahi University, Rajshahi 6205 (Bangladesh)
3. Department of Laboratory and Vascular Medicine, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8520 (Japan)
4. Department of Pharmacology, Faculty of Dentistry, Mahidol University, Bangkok 10400 (Thailand)

Endocannabinoids including anandamide (AEA) and 2-arachidonoylglycerol (2-AG) are important lipid mediators for immunosuppressive effects and for appropriate homeostasis via their G-protein-coupled cannabinoid (CB) receptors in mammalian organs and tissues, and may be involved in wound healing in some organs. The physiological roles of endocannabinoids in periodontal healing remain unknown. We observed upregulation of the expression of CB1/CB2 receptors localized on fibroblasts and macrophage-like cells in granulation tissue during wound healing in a wound-healing model in rats, as well as an increase in AEA levels in gingival crevicular fluid after periodontal surgery in human patients with periodontitis. In-vitro, the proliferation of human gingival fibroblasts (HGFs) by AEA was significantly attenuated by AM251 and AM630, which are selective antagonists of CB1 and CB2, respectively. CP55940 (CB1/CB2 agonist) induced phosphorylation of the extracellular-regulated kinases (ERK) 1/2, p38 mitogen-activated protein kinase (p38MAPK), and Akt in HGFs. Wound closure by CP55940 in an in-vitro scratch assay was significantly suppressed by inhibitors of MAP kinase kinase (MEK), p38MAPK, and phosphoinositol 3-kinase (PI3-K). These findings suggest that endocannabinoid system may have an important role in periodontal healing.

• OSTI ID: 22202475
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 4 Vol. 394; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
FIBROBLASTS
GRANULATION
GTP-ASES
HEALING
IN VITRO
LIPIDS
MACROPHAGES
ORGANS
PHOSPHORYLATION
PHOSPHOTRANSFERASES
RATS
RECEPTORS
WOUNDS